Cryopreservation of oil palm (Elaeis guineensis) zygotic embryos by controlled desiccation using saturated salt solutions /
The oil palm (Elaeis guineensis Jacq.) is Malaysia's golden crop as it contributes tremendously to our economy. In view of the importance of germlasm collection for breeding selection, various collection missions have been made. Currently these materials are being conserved in field gene banks,...
Uloženo v:
| Hlavní autor: | |
|---|---|
| Médium: | Diplomová práce Kniha |
| Jazyk: | English |
| Vydáno: |
2006.
|
| Tagy: |
Přidat tag
Žádné tagy, Buďte první, kdo otaguje tento záznam!
|
| LEADER | 03218nam a2200217 a 4500 | ||
|---|---|---|---|
| 001 | vtls026937429 | ||
| 003 | UPM | ||
| 005 | 20161022033031.0 | ||
| 008 | 061205s2006 my a 000 0 eng d | ||
| 039 | 9 | |a 201412261124 |b ss |c 200804172138 |d VLOAD |c 200701311147 |d suhaimi |c 200612261222 |d suhaimi |y 200612051526 |z afid | |
| 040 | |c UPM | ||
| 090 | 0 | 0 | |a FP 2006 13 |
| 100 | 0 | |a Greetha Arumugam. | |
| 245 | 1 | 0 | |a Cryopreservation of oil palm (Elaeis guineensis) zygotic embryos by controlled desiccation using saturated salt solutions / |c by Greetha a/p Arumugam. |
| 260 | |a 2006. | ||
| 300 | |a 67 leaves : |b ill. ; |c 30cm. | ||
| 502 | |a Project paper (B.S.Bi) - Universiti Putra Malaysia, 2006. | ||
| 520 | |a The oil palm (Elaeis guineensis Jacq.) is Malaysia's golden crop as it contributes tremendously to our economy. In view of the importance of germlasm collection for breeding selection, various collection missions have been made. Currently these materials are being conserved in field gene banks, as oil palm seeds are sensitive to desiccation and storage at low temperature. Field gene bank has constraints as they require plenty of land and are difficult to maintain. A potential alternative method is cryopreservation, which requires materials to be stored at ultra low temperature of liquid nitrogen (-196°C). Desiccation is an important factor in cryopreservation of oil palm zygotic embryos. This study optimizes the desiccation protocol with saturated salt solutions. First part of the study, consisted of desiccation oil palm embryo in the air current of laminar fair air flow cabinet to 5 desiccation periods (0, 1, 2, 3 and 4 hours) and subjected to liquid nitrogen exposure for 16 hours. The % moisture content of embryos calculated and cultured in MS medium to evaluate % viability and % survival after 4 and 8 weeks. In the second part of study, the naked oil palm embryos wwere desiccated in a controlled environment using various saturated salt solutions, which gives different relative humidity. For suitable saturated salt solutions (62%, 45%, 23% and 8%) were chosen as desiccant at the end for the next evaluation. Third part of the study embryos were desiccated in saturated salt solutions and cultured in MS medium and percentage of survival and viability evaluated upon exposure to liquid nitrogen after 4 and 8 weeks respectively. Results show that moisture content is an inportant factor in the survival of oil palm embryos. Desiccation in the air current of laminar air flow aroud ~15% gives the highest survival of ~70%. Zygotic embryos of oil palm required between 6 to 8 hours for equilibration to occur for salts which had relative humidities below 75%. Relative humidity above that level is injurious to the cells of the embryos. Survival after incubation in the designated salts solutions with and without exposure to liquid nitrogen, were relatively low (`55%) but precise moisture was obtainable through this method. However, the use of direct desiccation method gave higher survival is suitable for cryopreservation. | ||
| 942 | |2 lcc |c 50003 | ||
| 999 | |c 240154 |d 240154 | ||
| 952 | |0 0 |1 0 |4 0 |6 FP2006 00013 |7 0 |9 364449 |a 10000 |b 10000 |c 50003 |d 2016-10-22 |o FP 2006 13 |p 1000568060 |r 2016-10-22 |w 2016-10-22 |y 50003 | ||