Gene cloning and manipulation - Howe, Christopher (Cambridge ; New York : Cambridge University Press, 2007)
The tools for the job -- Polymerase chain reaction -- Simple cloning -- Other vector systems for E. coli -- Making libraries -- Screening libraries -- Modification and mutagenesis -- Use of cloned DNA -- Using other organisms.
Keywords: Molecular cloning; Cloning, Molecular -- methods; Escherichia coli -- Genetics; Gene Library; Transformation, Genetic