TY - THES TY - BOOK T1 - Prevalence of mannheimia haemolytica and pasteurella multodica in goats from selected farms in Selangor A1 - Nuraini Simaa Alway LA - English UL - http://discoverylib.upm.edu.my/discovery/Record/47008 AB - Mannheimia haemlytica and Pasteurella mutocida are natural inhabitants of upper respiratory tract of healthy animals and will cause pneumonic pasteurellosis in stressed or infected animals. Healthy animals are able to control the multiplication of these bacteria and the inhaled bacteria, if they migrate to the lungs, will be cleared by hosts’ defense mechanisms. Stress and other infections will cause the breakdown of hosts’ defense mechanisms. These will lead to multiplication of the bacteria and colonization of the lungs. Outbreaks of pneumonia will occurs in 10 to 14 days post stress exposure. These bacteria are gram negative, facultative anaerobes and have rod-shaped morphology. The special characteristic of these bacteria are they will show bipolar staining characteristic under Giemsa and Wright’s stain. Mannheimia haemolytica is haemolytic on blood agar and O-nitrophenyl-β,D-glactopyranoside (ONPG) positive while Pasteurella mutocida is non-haemolytic on blood agar and ONPG negative. Nasopharyngeal swab samples were taken from 96 goats in 4 farms in Selangor and bacterial isolation and identification were carried out. Presumptive isolated were identified by biochemical test. Out of 96 samples, 3 were positive for Mannheimia haemolytica and 11 were positive for Pasteurella multicida. This gives a 3.13% and 11.46% prevalence rate for Mannheimia haemolytica and Pasteurella mutocida respectively. The antibiotic sensitivity tests done on both the isolates showed 29% of were resistant to streptomycin and 21% were resistant to compound sulfonamide. All isolates were sensitive to ampicillin and amoxicillin-clavulanic acid and 93% were sensitive to oxytetracycline and enrofloxacin. Even with the low prevalence of Mannheimia haemolytica and Pasteurella mutocida, it is important to control and prevent their infection and also to prevent further development of antimicrobial resistance as the disease associated with these bacteria have high economic impact. CN - FPV 2012 42 ER -