TY  - THES
TY  - BOOK
T1  - Gross and histopathological changes associated with dose dependent infection by Corynebacterium pseudotuberculosis inmice via oral route inoculation
A1  - Tuba Thabitah Abdullah Tahir
LA  - English
UL  - http://discoverylib.upm.edu.my/discovery/Record/51583
AB  - Carynebacterium pseudotuberculosisis a facultative, rod shaped, gram-positive bacteria which is a causative agent of caseous lymphadenitis (CLA).   CLA is a chronic granulomatous infectious disease that is characterized by the formulation of absecesses, typically located in superficial lymph nodes and lungs.  Difficulties in early clinical identification of CLA uinfectes animals have limited the effectiveness of controlling and eradicating this disease.  This study was conducted to acquire a better way of understanding the pathogenesis and compare the lesions severity with dose dependent of CLA through mice model.  40 healthy male mice were divided equally into 4 groups, where the first group of mice were orally inoculated with 0.4 mL of sterile phosphate buffer solution (PBS), pH 7, the second, third and fourth groups of mice were orally inoculated with 0.4 mL of 10 3, 10 5 and 10 7 colony forming unit (cfu) of C. pseudotuberculosis respectively.  Gross and histopathological changes in visceral organs were compared between the 3 different diseased groups and non-diseases group within the period of 240 hours or 10 days of post inoculation. Data was analyzed using the SPSS version 19.  Results of this study showed tha there were significant (p<0.05)  histopathological changes observed in the visceral organs  (lung, liver and kidney) between 3 diseased groups and non-diseased group.  There was significant (p<0.05) between dose 10  5 and 10 7 but there was no significant different (>0.05) between dose 10 5 and 10 7.  The lesions severity with dose 10 3 were normal to mild, while dose 10 5 and 10 7 mild to moderate severity where the visceral organs had severe congestion and increased vascularization, micro-abscesses formation, infiltration of neutrophils and macrophages, tubercular granulomas, necrosis and early signs of degeneration.
CN  - FPV 2013 23
ER  -