TY - THES TY - BOOK T1 - Isolation and characterization of antimicrobial resistant Escherichia coli and Enterococcus spp. from four farms of village chickens in Hulu Langat A1 - Muhammad Syazani Japri. LA - English YR - 2019 UL - http://discoverylib.upm.edu.my/discovery/Record/575842 AB - In the poultry industry, the use of antimicrobials as growth promoters, feed additives and prophylaxis are considered to contribute to antimicrobial resistance (AMR) in chickens, which may pose as a risk to humans via the food production line. As such it has triggered awareness among the public, leading to change in lifestyles and food, including sourcing foods of animal origin raised freely without the usage of antimicrobials of other chemicals. Therefore this study aimed to determine if AMR is present in two commensal species in the intestine of chickens; Escherichia coli and Enterococcus spp. in village chickens. In this study, 60 cloacal swabs were acquired from village chickens from four farms located in the Hulu Langat district, Selangor. Escherichia coli and Enterococcus spp. were isolated from the sample with the overall prevalence of 89.3% and 37.5%, respectively. Kirby-Baur disc diffusion method was used to obtain the antibiogram profile of the E. coli and Enterococcus spp. isolates, E. coli isolates were resistant to tetracycline (78%), ampicillin (56%), amoxicillin (52%), streptomycin (38%), chioramphenicol (34%), cephalexin (28%), enrofloxacin (24%), gentamycin (8%) and aztreonam (2%), but susceptible to meropenem. Enterococcus spp. were resistant to tetracycline (100%), norfloxacin (72%), enrofloxacin (52%) gentamyein (33%), ciprofloxacin (33%), ampicillin (29%), and amoxicillin (10%), but susceptible to vancomycin. The percentage of multiple drug resistant isolates for E. coli and Enterococcus spp. were 59% and 66.7% respectively. Therefore educating the farmers on multiple drug resistance and AMR should be of priority and be addressed by the One Health approach involving veterinarians, policy makers, pharmaceutical companies, among others, to help curb the spread of AMR to humans, other animal species and the environment. CN - FPV 2019 59 ER -