TY - THES TY - BOOK T1 - Evaluation of essential oil of garlic as prophylactic agent on Aeromonas hydrophila infection in red tilapia with the presence of stress factor A1 - Nesea Janoh. LA - English YR - 2019 UL - http://discoverylib.upm.edu.my/discovery/Record/575844 AB - The present study aimed to investigate the effects of duration dietary exposure of garlic essential oil (Allium sativum) on its prophylactic properties against Aeromonas hydrophila infection in Red hybrid tilapia fingerlings with the presence of stress factor. 0.1mL of garlic essential oil was mixed with every 100 g of fish commercial feed. There were 3 group; group fed with garlic oil for 5 days and 10 days, and the group fed with normal commercial fish feed for 10 days. Each group was further divided into two groups; with and without heat stress. After the fish were challenged with A. hydrophila, clinical signs and mortality were monitored. At the same time, the water temperature in groups challenged with heat stress was maintained at 35˚C. After 7 days, organs such as gills, kidney and spleen were sampled for lesion scoring. Lesions such as congestion, necrosis, vacuolation and inflammation were evaluated. The result showed a mean lesion score of the spleen was not significantly different (p<0.05) higher in groups with heat stress than the groups without heat stress regardless of the different in the duration of garlic oil exposure. Besides, the mean lesion score in groups fed with garlic oil for 10 days was higher than the group fed with garlic oil for 5 days. Heat factor influenced the severity of infection due to long-term elevated cortisol that causing immunosuppression. In conclusion, group fed with garlic essential oil for 10 days with elucidated better effect in enhancing the resistance of Red hybrid tilapia fingerlings towards A. hydrophila infection as the lesion score was the lowest among other groups. CN - FPV 2019 61 ER -