TY - GEN TY - GEN T1 - Shotgun Proteomics Methods and Protocols T2 - Methods in Molecular Biology, A2 - Martins-de-Souza, Daniel. A2 - Martins-de-Souza, Daniel. LA - English PP - New York, NY PB - Springer New York : Imprint: Humana YR - 2014 ED - 1st ed. 2014. UL - http://discoverylib.upm.edu.my/discovery/Record/978-1-4939-0685-7 AB - Shotgun Proteomics: Methods and Protocols serves as a vital collection of protocols through which thousands of proteins can be simultaneously identified, quantified, and characterized in a high throughput manner.  Beginning with the history of proteomics centered on the vital role of mass spectrometry in its development, this detailed volume continues with chapters on sample pre-fractionation, in vivo and in vitro stable isotope labeling, label-free proteomics, informatics, protein-protein interactions, targeted proteomics, and post-translational modifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and comprehensive, Shotgun Proteomics: Methods and Protocols is an ideal and up-to-date guide for researchers seeking to understand the proteome of any given species. OP - 492 CN - QD431-431.7 SN - 9781493906857 KW - Proteins . KW - Proteomics. KW - Protein Science. ER -