Bacterial Transcriptional Control Methods and Protocols /
This volume is designed to be a resource of proven techniques and approaches for probing the activities of bacterial, eukaryotic, and archaeal RNA polymerases. This book features a collection of in vitro and in vivo technologies that will permit researchers to purify and probe the position and stabi...
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| Korporacja: | |
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| Kolejni autorzy: | , |
| Format: | Elektroniczne E-book |
| Język: | English |
| Wydane: |
New York, NY :
Springer New York : Imprint: Humana,
2015.
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| Wydanie: | 1st ed. 2015. |
| Seria: | Methods in Molecular Biology,
1276 |
| Hasła przedmiotowe: | |
| Dostęp online: | https://doi.org/10.1007/978-1-4939-2392-2 |
| Etykiety: |
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Spis treści:
- Mapping the Escherichia coli Transcription Elongation Complex with Exonuclease III
- Purification of Bacterial RNA Polymerase: Tools and Protocols
- Monitoring Translocation of Multisubunit RNA Polymerase Along the DNA with Fluorescent Base Analogues
- In vitro and in vivo Methodologies for Studying the Sigma54-dependent Transcription
- Methods for the Assembly and Analysis of in vitro Transcription-coupled-to-translation Systems
- Site-specific Incorporation of Probes into RNA Polymerase by Unnatural-amino-acid Mutagenesis and Staudinger-Bertozzi Ligation
- Reconstitution of Factor-dependent, Promoter Proximal Pausing in Drosophila Nuclear Extracts
- Direct Competition Assay for Transcription Fidelity
- Single-stranded DNA Aptamers for Functional Probing of Bacterial RNA Polymerase
- Biochemical Analysis of Transcription Termination by RNA Polymerase III from Yeast Saccharomyces cerevisiae
- Use of RNA Polymerase Molecular Beacon Assay to Measure RNA Polymerase Interactions with Model Promoter Fragments
- Preparation of cDNA Libraries for High-throughput RNA Sequencing Analysis of RNA 5’ Ends
- In situ Footprinting of E. coli Transcription Elongation Complex with Chloroacetaldehyde
- Using Solutes and Kinetics to Probe Large Conformational Changes in the Steps of Transcription Initiation
- Manipulating Archaeal Systems to Permit Analyses of Transcription Elongation-termination Decisions in vitro
- Purification of Active RNA Polymerase I from Yeast
- Transcription in Archaea: preparation of Methanocaldococcus jannaschii Transcription Machinery
- Transcription in Archaea: in vitro Transcription Assays for mjRNAP
- Experimental Analysis of hFACT Action during Pol II Transcription in vitro
- ChIP-seq for Genome-scale Analysis of Bacterial DNA-binding Proteins.