Methods in Mouse Atherosclerosis

This volume reviews available mouse models of atherosclerosis, basic aspects of disease initiation and progression, and the visualization of atherosclerotic lesions with invasive and non-invasive techniques that are widely used both in basic research and in the clinic. The chapters catalogue a wide...

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Collectivité auteur: SpringerLink (Online service)
Autres auteurs: Andrés, Vicente. (Éditeur intellectuel, http://id.loc.gov/vocabulary/relators/edt), Dorado, Beatriz. (Éditeur intellectuel, http://id.loc.gov/vocabulary/relators/edt)
Format: Électronique eBook
Langue:English
Publié: New York, NY : Springer New York : Imprint: Humana, 2015.
Édition:1st ed. 2015.
Collection:Methods in Molecular Biology, 1339
Sujets:
Accès en ligne:https://doi.org/10.1007/978-1-4939-2929-0
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Table des matières:
  • Use of Mouse Models in Atherosclerosis Research
  • Cytokines and Immune Responses in Murine Atherosclerosis
  • Intravital Microscopy for Atherosclerosis Research
  • Non-invasive Molecular Imaging of Mouse Atherosclerosis
  • Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root
  • Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages
  • Isolation of Mouse Primary Aortic Endothelial Cells by Selection with Specific Antibodies
  • Isolation and Culture of Aortic Smooth Muscle Cells and In Vitro Calcification Assay
  • Immunostaining of Macrophages, Endothelial Cells, and Smooth Muscle Cells in the Atherosclerotic Mouse Aorta
  • Immunostaining of Lymphocytes In Mouse Atherosclerotic Plaque
  • Flow Cytometric Analysis of Immune Cells Within Murine Aorta
  • In Vitro Differentiation of Naïve CD4+ T cells: A Tool for Understanding the Development of Atherosclerosis
  • Quantification of Apoptosis in Mouse Atherosclerotic Lesions
  • Quantification of Cellular Proliferation in Mouse Atherosclerotic Lesions
  • Quantification of in vitro Macrophage Cholesterol Efflux and in vivo Macrophage-Specific Reverse Cholesterol Transport
  • In vitro Macrophage Phagocytosis Assay
  • Glucose and Insulin Tolerance Tests in the Mouse
  • Wire Myography to Study Vascular Tone and Vascular Structure of Isolated Mouse Arteries
  • Generation of Aorta Transcript Atlases of Wild-type and Apolipoprotein E-null Mice by Laser Capture Microdissection-based mRNA Expression Microarrays
  • Analysis of Gene and Protein Expression In Atherosclerotic Mouse Aorta By Western Blot and Quantitative Real-Time PCR
  • Bone Marrow Transplantation in Mice to Study the Role of Hematopoietic Cells in Atherosclerosis
  • Tandem Stenosis to Induce Atherosclerotic Plaque Instability in the Mouse
  • Detection of Intraplaque Hemorrhage in Mouse Atherosclerotic Lesions
  • Mechanical Stabilization of Mouse Carotid Artery for in vivo intravital Microscopy Imaging of Atherogenesis
  • Intravital Microscopy in the Cremaster Muscle Microcirculation for Endothelial Dysfunction Studies. - Fluorescent Molecular Tomography for in vivo Imaging of Mouse Atherosclerosis
  • In vivo 18F-FDG-PET Imaging in Mouse Atherosclerosis
  • Magnetic Resonance Imaging of the Atherosclerotic Mouse Aorta.