Chemokine Protocols

In the past decade research has established the biological importance of chemokines: they play a major role in leukocyte trafficking, in the recruitment of leukocytes to inflammatory sites, and are coreceptors along with CD4 for HIV cell entry. In Chemokine Protocols, expert investigators describe i...

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Tác giả của công ty: SpringerLink (Online service)
Tác giả khác: Proudfoot, Amanda E.I. (Biên tập viên, http://id.loc.gov/vocabulary/relators/edt), Wells, Timothy N.C. (Biên tập viên, http://id.loc.gov/vocabulary/relators/edt), Power, Christine. (Biên tập viên, http://id.loc.gov/vocabulary/relators/edt)
Định dạng: Điện tử eBook
Ngôn ngữ:English
Được phát hành: Totowa, NJ : Humana Press : Imprint: Humana, 2000.
Phiên bản:1st ed. 2000.
Loạt:Methods in Molecular Biology, 138
Những chủ đề:
Truy cập trực tuyến:https://doi.org/10.1385/1592590586
Các nhãn: Thêm thẻ
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Mục lục:
  • Purification of Chemokines from Natural Sources
  • Cloning of Novel Chemokines Using a Signal Sequence Trap Method
  • Chemokine Expression in Insect Cells
  • Expression of Chemokines in Escherichia coli
  • Expression of Chemokines in the Periplasmic Space of E. coli
  • Synthesis of Chemokines
  • Identification of Novel Chemokines From Expressed Sequence Tag Databases
  • Purification of Recombinant Chemokines from E. coli
  • Chemokine Receptor Cloning
  • Generation of Stable Cell Lines Expressing Chemokine Receptors
  • Modified Microchemotaxis Assays
  • Transwell Chemotaxis
  • Endothelial Cell Chemotaxis Assays
  • Radiolabeled Chemokine Binding Assays
  • Scintillation Proximity Binding Assay
  • Calcium Mobilization
  • Actin Polymerization
  • Reactive Oxygen Release
  • Histamine Release
  • Measurement of Phosphoinositide 3-Kinase Activity
  • Glycosaminoglycan Binding Assays
  • CFU-A Assay for Measurement of the Antiproliferative Effects of Chemokines on Murine Early Hemopoietic Progenitors
  • Downmodulation and Recycling of Chemokine Receptors
  • Analysis of Chemokine Receptor Endocytosis and Recycling
  • Chemokine Inhibition of HIV Infection
  • 26The Production of Chemokine Specific Monoclonal Antibodies
  • Monoclonal Antibodies to Chemokine Receptors
  • Targeted Expression of Chemokines In vivo
  • Chemokine Knockout Mice
  • Chemokine Receptor Knockout Mice
  • Measurement of Eosinophil Accumulation In vivo
  • Murine Model of Allergic Lung Inflammation
  • Murine Models of Airway Inflammation
  • Rat Models of Respiratory Inflammation
  • Murine Model of Crescentic Nephritis
  • Rabbit Models of Pneumonia, Peritoneal Sepsis, and Lung Injury.