Colorectal Cancer Methods and Protocols /
Colorectal cancer is a common and highly lethal cancer once spread whose treatment is improving as we gain a better understanding of its development. In Colorectal Cancer: Methods and Protocols, Steven M. Powell, MD, has assembled a hands-on collection of cutting-edge molecular methods for studying...
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| Korporativní autor: | |
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| Další autoři: | |
| Médium: | Elektronický zdroj E-kniha |
| Jazyk: | English |
| Vydáno: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2001.
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| Vydání: | 1st ed. 2001. |
| Edice: | Methods in Molecular Medicine,
50 |
| Témata: | |
| On-line přístup: | https://doi.org/10.1385/1592590845 |
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Obsah:
- Microdissection of Histologic Sections
- Isolation of a Purified Epithelial Cell Population from Human Colon
- Xenografting Human Colon Cancers
- Comparative Genomic Hybridization Technique
- Fluorescence In Situ Hybridization
- Microsatellite Analysis to Assess Chromosome 18q Status in Colorectal Cancer
- The SURF Technique
- Microsatellite Instability Testing
- Immunohistochemical Analysis for hMLH1 and hMSH2 Expression in Colorectal Cancer
- Mutation Detection in Colorectal Cancers
- Mutation Detection inTGF-? Receptors
- Direct Analysis for Familial Adenomatous Polyposis Mutations
- I1307K Mutation Detection by Allele-Specific PCR in Familial Colorectal Cancer
- Microsatellite Analysis of the Insulin-Like Growth Factor II Receptor in Colorectal Carcinomas
- Molecular Detection of Smad2/Smad4 Alterations in Colorectal Tumors
- Direct Sequencing for Juvenile Polyposis Gene SMAD4/DPC4 Mutations
- 17 Direct Sequencing for Peutz-Jeghers Gene LKB1 (STK11) Mutations
- 18 Direct Sequencing for Cowden Syndrome Gene PTEN (MMAC1) Mutations
- Genetic Analysis Using Microarrays
- Cclooxygenase-2 (COX-2) Protein Expression by Western Blotting
- The Immunohistochemical Method
- Immunohistochemical Detection and Quantitation of Cell Surface Receptors for Prostanoids
- Adhesion Signaling Through Integrins
- Electrophoretic Mobility Shift Assay (EMSA)
- Transfection Assays for Transformation of Model Colonic Cell Lines
- Assessment of Intestinal Stem Cell Survival Using the Microcolony Formation Assay
- Gene Transfer into the Colonic Mucosa.



