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Hematopoietic Stem Cell Protocols

Hematopoietic stem cells have an enormous clinical importance for bone marrow transplantation, hematopoietic gene therapy, solid organ transplantation, and somatic tissue regeneration. In Hematopoietic Stem Cell Protocols, a group of leading stem-cell investigators describe in detail the key laborat...

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书目详细资料
企业作者: SpringerLink (Online service)
其他作者: Klug, Christopher A. (Editor, http://id.loc.gov/vocabulary/relators/edt), Jordan, Craig T. (Editor, http://id.loc.gov/vocabulary/relators/edt)
格式: 电子 电子书
语言:English
出版: Totowa, NJ : Humana Press : Imprint: Humana, 2002.
版:1st ed. 2002.
丛编:Methods in Molecular Medicine, 63
主题:
Hematology.
在线阅读:https://doi.org/10.1385/159259140X
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书本目录:
  • Isolation and Analysis of Hematopoietic Stem Cells from Mouse Embryos
  • The Purification of Mouse Hematopoietic Stem Cells at Sequential Stages of Maturation
  • Flow Cytometry and Immunoselection of Human Stem Cells
  • Method for Purification of Human Hematopoietic Stem Cells by Flow Cytometry
  • Noninvasive Measurement of Hematopoietic Stem Cell Cycling and Turnover by In Vivo Bromodeoxyuridine Incorporation
  • Isolation and Characterization of Primitive Hematopoietic Cells Based on Their Position in the Cell Cycle
  • Hematopoietic Colony-Forming Cells
  • Long-Term Culture-Initiating Cell Assays for Human and Murine Cells
  • The Cobblestone-Area-Forming Cell Assay
  • CFU-S
  • Intrathymic Injection for Analysis of T-Cell Progenitor Activity
  • Quantitation of Murine and Human Hematopoietic Stem Cells by Limiting-Dilution Analysis in Competitively Repopulated Hosts
  • Ex Vivo Expansion of Human and Murine Hematopoietic Stem Cells
  • Hematopoietic Development of ES Cells in Culture
  • Genetic Modification of Murine Hematopoietic Stem Cells by Retroviruses
  • Retroviral Transduction of FACS-Purified Hematopoietic Stem Cells
  • Retroviral-Mediated Transduction and Clonal Integration Analysis of Human Hematopoietic Stem and Progenitor Cells
  • Production of Lentiviral Vector Supernatants and Transduction of Cellular Targets
  • Reverse Transcriptase-PCR Analysis of Gene Expression in Hematopoietic Stem Cells
  • Identification of Differentially Expressed Genes in Sorted Cell Populations by Two-Dimensional Gene-Expression Fingerprinting.

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