TY - GEN TY - GEN T1 - Protein Sequencing Protocols T2 - Methods in Molecular Biology, A2 - Smith, Bryan John. A2 - Smith, Bryan John. LA - English PP - Totowa, NJ PB - Humana Press : Imprint: Humana YR - 2003 ED - 2nd ed. 2003. UL - http://discoverylib.upm.edu.my/discovery/Record/978-1-59259-342-2 AB - Bryan John Smith updates his much-acclaimed first edition of Protein Sequencing Protocols with new and refreshed techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current importance of biopharmaceuticals, but also offers a guide to the analysis of protein sequence information via the powerful new tools of bioinformatics. Each readily reproducible protocol contains detailed step-by-step instructions to ensure successful experimental results, along with notes on troubleshooting and how to avoid pitfalls. Valuable appendices summarize changes in mass arising from protein modification, as well as consensus sequences for protein modification. Comprehensive and up-to-date, Protein Sequencing Protocols, Second Edition, provides both novice and expert investigators alike a ready source of easy-to-follow protocols that simplify choosing the most appropriate method for protein sequence determination and thus help assure robust results. OP - 493 CN - QH345 SN - 9781592593422 KW - Biochemistry. KW - Biochemistry, general. ER -