Combinatorial Peptide Library Protocols

Shmuel Cabilly presents in Combinatorial Peptide Library Protocols a collection of new and unique techniques for the construction and use of peptide libraries. These powerful methods-often detailed here by their pioneers-include protocols for the chemical synthesis of peptide libraries, for construc...

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Autor corporatiu: SpringerLink (Online service)
Altres autors: Cabilly, Shmuel. (Editor, http://id.loc.gov/vocabulary/relators/edt)
Format: Electrònic eBook
Idioma:English
Publicat: Totowa, NJ : Humana Press : Imprint: Humana, 1998.
Edició:1st ed. 1998.
Periòdiques:Methods in Molecular Biology, 87
Matèries:
Accés en línia:https://doi.org/10.1385/0896033929
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Taula de continguts:
  • Synthesis of a One-Bead One-Compound Combinatorial Peptide Library
  • Enzyme-Linked Calorimetric Screening of a One-Bead One-Compound Combinatorial Library
  • Synthesis and Screening of Positional Scanning Combinatorial Libraries
  • Synthesis and Screening of Peptide Libraries on Continuous Cellulose Membrane Supports
  • Peralkylation
  • to Combinatorial Solid-Phase Assays for Enzyme Activity and Inhibition
  • Preparation of Biocompatible Resins for Library Syntheses
  • Intramolecular Fluorescence-Quenched Substrate Libraries
  • The Solid-Phase Enzyme Inhibitor Library Assay
  • Determination of Peptide Substrate Motifs for Protein Kinases Using a “One-Bead One-Compound” Combinatorial Library Approach
  • The Use of Peptide Library for the Determination of Kinase Peptide Substrates
  • Analysis of Protein Kinase Substrate Specificity by the Use of Peptide Libraries on Cellulose Paper (SPOT-Method)
  • Generation of Multiuse Peptide Libraries for Functional Screenings
  • Functional Screening of Multiuse Peptide Libraries Using Melanophore Bioassay
  • The Basic Structure of Filamentous Phage and Its Use in the Display of Combinatorial Peptide Libraries
  • Construction and Use of a 20-mer Phage Display Epitope Library
  • Construction of Disulfide-Constrained Random Peptide Libraries Displayed on Phage Coat Protein VIII
  • Conformational Mimicry Through Random Constraints Plus Affinity Selection
  • The Use of Combinatorial Libraries to Identify Ligands That Interact with Surface Receptors in Living Cells
  • Screening Phage Display Peptide Libraries on Nitrocellulose Membranes
  • Identification of Disease-Specific Epitopes
  • Identification of Peptide Ligands for the Antigen Binding Receptor Expressed on Human B-Cell Lymphomas
  • Major Histocompatibility Complex Allele-Specific Peptide Libraries and Identification of T-Cell Mimotopes
  • Phage Display of Peptide Libraries on Protein Scaffolds
  • Displaying Libraries of Conformationally Constrained Peptides on the Surface of Escherichia coli as Flagellin Fusions
  • Combinatorial Peptide Library as an Immunogen.