2-D Proteome Analysis Protocols
In 2-D Gel Proteome Analysis Protocols, Andrew Link and his expert collaborators take today's researchers step-by-step through the complete process of doing proteomics. With easy-to-follow instructions, complete with many helpful hints and explanations, leading investigators and pioneers in the...
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| 格式: | 电子 电子书 |
| 语言: | English |
| 出版: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1999.
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| 版: | 1st ed. 1999. |
| 丛编: | Methods in Molecular Biology,
112 |
| 主题: | |
| 在线阅读: | https://doi.org/10.1385/1592595847 |
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书本目录:
- 2-D Protein Gel Electrophoresis
- Solubilization of Proteins in 2-D Electrophoresis
- Preparation of Escherichia coli Samples for 2-D Gel Analysis
- Preparing 2-D Protein Extracts from Yeast
- 2-D Protein Extracts from Drosophila melanogaster
- Preparing 2-D Protein Extracts from Caenorhabditis elegans
- Eukaryotic Cell Labeling and Preparation for 2-D
- Differential Detergent Fractionation of Eukaryotic Cells
- Fractionated Extraction of Total Tissue Proteins from Mouse and Human for 2-D Electrophoresis
- Preparation and Solubilization of Body Fluids for 2-D
- 2-D Electrophoresis of Plant Proteins
- Quantifying Protein in 2-D PAGE Solubilization Buffers
- Measuring the Radioactivity of 2-D Protein Extracts
- Advantages and Disadvantages of Carrier Ampholyte IEF
- 2-D Electrophoresis Using Carrier Ampholytes in the First Dimension (IEF)
- Nonequilibrium pH Gel Electrophoresis (NEPHGE)
- High-Resolution, 2-D Protein Electrophoresis Using Nondedicated Equipment
- Large-Gel 2-D Electrophoresis
- Advantages of Immobilized pH Gradients
- Casting Immobilized pH Gradients (IPGs)
- Analytical IPG-Dalt
- IPG-Dalt of Very Alkaline Proteins
- Running Preparative Carrier Ampholyte and Immobilized pH Gradient IEF Gels for 2-D
- In-Gel Sample Rehydration of Immobilized pH Gradient
- High-Resolution, IPG-Based, Mini Two-Dimensional Gel Electrophoresis
- Horizontal SDS-PAGE for IPG-Dalt
- Casting and Running Vertical Slab-Gel Electrophoresis for 2D-PAGE
- Nonreducing 2-D Polyacrylamide Gel Electrophoresis
- 2-D Diagonal Gel Electrophoresis
- 2-D Phosphopeptide Mapping
- Internal Standards for 2-D
- Autoradiography of 2-D Gels
- Double-Label Analysis
- Silver Staining of 2-D Electrophoresis Gels
- Staining of Preparative 2-D Gels
- Electroblotting of Proteins from 2-D Polyacrylamide Gels
- Detection of Total Proteins on Western Blots of 2-D Polyacrylamide Gels
- Protein Detection Using Reversible Metal Chelate Stains
- Glycoprotein Detection of 2-D Separated Proteins
- Image Acquisition in 2-D Electrophoresis
- Computer Analysis of 2-D Images
- 2-D Databases on the World Wide Web
- Comparing 2-D Electrophoretic Gels Across Internet Databases
- Constructing a 2-D Database for the World Wide Web
- Absolute Quantitation of 2-D Protein Spots
- Generating a Bacterial Genome Inventory
- Immunoaffinity Identification of 2-DE Separated Proteins
- 2-DE Spot Amino Acid Analysis with 9-Fluorenylmethyl Chloroformate
- N-Terminal Amino Acid Sequencing of 2-DE Spots
- Characterizing Proteins from 2-DE Gels by Internal Sequence Analysis of Peptide Fragments
- Obtaining Molecular Weights of Proteins and Their Cleavage Products by Directly Combining Gel Electrophoresis with Mass Spectrometry
- Identification of Proteins by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using Peptide and Fragment Ion Masses
- Sample Preparation Methods for Mass Spectrometric Peptide Mapping Directly from 2-DE Gels
- Protein Identification and Analysis Tools in the ExPASy Server
- Automated Protein Identification Using Microcolumn Liquid Chromatography-Tandem Mass Spectrometry
- Peptide Sequencing of 2-DE Gel-Isolated Proteins by Nanoelectrospray Tandem Mass Spectrometry.