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Patch-Clamp Applications and Protocols

E. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, they used firepolished micropipets with a tip diameter of 3-5 pm to record currents from a small patch of the membranbe of sk- etal muscles, thereby dec...

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企业作者: SpringerLink (Online service)
其他作者: Boulton, Alan A. (Editor, http://id.loc.gov/vocabulary/relators/edt), Baker, Glen B. (Editor, http://id.loc.gov/vocabulary/relators/edt), Walz, Wolfgang. (Editor, http://id.loc.gov/vocabulary/relators/edt)
格式: 电子 电子书
语言:English
出版: Totowa, NJ : Humana Press : Imprint: Humana, 1995.
版:1st ed. 1995.
丛编:Neuromethods, 26
主题:
Neurosciences.
在线阅读:https://doi.org/10.1385/0896033112
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书本目录:
  • Technology of Patch- Clamp Electrodes
  • Whole-Cell Patch-Clamp Recordings
  • Pressure/Patch-Clamp Methods
  • Cell-Free Ion-Channel Recording
  • Perfusion of Patch Pipets
  • Concentration Clamp Technique
  • Perforated Patch-Clamp Technique
  • The Loose Patch Voltage Clamp Technique
  • Patch-Clamp Recording and RT-PCR on Single Cells
  • Patch-Clamp Technique in Brain Slices
  • Xenopus Oocyte Microinjection and Ion-Channel Expression.

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