Cell Cycle Checkpoint Control Protocols
In recent years, cell cycle checkpoints, cellular mechanisms that control the cell cycle and ensure genomic stability, have emerged as significant factors in carcinogenesis and in cancer cells. In Cell Cycle Checkpoint Control Protocols, leading investigators present their best methodologies to prob...
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| Другие авторы: | |
| Формат: | Электронный ресурс eКнига |
| Язык: | English |
| Опубликовано: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2004.
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| Редактирование: | 1st ed. 2004. |
| Серии: | Methods in Molecular Biology,
241 |
| Предметы: | |
| Online-ссылка: | https://doi.org/10.1385/1592596460 |
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Оглавление:
- Induction and Detection of Changes in cell Cycle Progression
- Methods to Induce Cell Cycle Checkpoints
- Methods for Synchronizing Mammalian Cells
- Enrichment of Cells in Different Phases of the Cell Cycle by Centrifugal Elutriation
- Analysis of the Mammalian Cell Cycle by Flow Cytometry
- Methods for Detecting Cells in S Phase
- Yeast Cell Synchronization
- Analysis of the Budding Yeast Saccharomyces cerevisiae Cell Cycle by Morphological Criteria and Flow Cytometry
- Analysis of the Fission Yeast Schizosaccharomyces pombe Cell Cycle
- Analysis of Genes Involved in Checkpoint Control
- Strategies to Isolate Evolutionarily Conserved Cell Cycle Regulatory Genes
- Microarray Approaches for Analysis of Cell Cycle Regulatory Genes
- Using the Yeast Genome-Wide Gene-Deletion Collection for Systematic Genetic Screens
- Gene Targeting in Cultured Human Cells
- Use of In Vivo Gap Repair for Isolation of Mutant Alleles of a Checkpoint Gene
- In Vitro Mutagenesis to Define Functional Domains
- Use of Gene Overexpression to Assess Function in Cell Cycle Control
- Histone Acetylation/Deacetylation As a Regulator of Cell Cycle Gene Expression
- Analysis of Proteins Involved in Checkpoint Control
- Cataloging Proteins in Cell Cycle Control
- Multidimensional Proteomic Analysis of Proteolytic Pathways Involved in Cell Cycle Control
- Purification and Identification of Protein Complexes That Control the Cell Cycle
- Xenopus Cell-Free Extracts to Study DNA Damage Checkpoints
- Protein-Protein Interactions
- Detection of Kinase and Phosphatase Activities
- Monitoring Changes in the Subcellular Location of Proteins in S. cerevisiae
- Chromosomes and the Cell Cycle
- Chromosomal Changes and Cell Cycle Checkpoints in Mammalian Cells
- Detecting the Influence of Cell Cycle Regulatory Proteins on Human Telomeres
- Monitoring Spindle Assembly and Disassembly in Yeast by Indirect Immunofluorescence.