Cytokine Protocols
Because cytokines regulate many different bodily functions in living organisms, mainly at the level of cell-cell communications, there is great interest in understanding their mode of action. In Cytokine Protocols, established researchers, physicians, and clinicians present their best biochemical, c...
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| Format: | Electronic eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2004.
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| Edition: | 1st ed. 2004. |
| Series: | Methods in Molecular Biology,
249 |
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| Online Access: | https://doi.org/10.1385/1592596673 |
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Table of Contents:
- Large-Scale Generation of Plasmids that Express Type I Interferon
- Identification of trans-Acting Factors by Electrophoretic Mobility Shift Assay
- Modulation of the Interferon-? Signal by Transfection of Cells with an Antisense-RNA Expressing Vector
- Competitive RT-PCR to Quantify Small Amounts of mRNA
- In SituHybridization for Cytokines in Human Tissue Biopsies
- Purification, Identification, and Synthesis of Chemokines
- Receptor Isolation and Characterization
- Crystallization of Cytokine-Receptor Complexes
- Biosensor Analysis of Receptor-Ligand Interactions
- Analysis of SH2 Ligands and Identification of Sites of Interaction
- Assays for Antiviral Activity
- Assays for Cytotoxicity
- Assays for Chemotaxis
- In Vitro and In Vivo Assays for High-Molecular-Weight Pegylated Muteins of Granulocyte Colony-Stimulating Factor
- Development of a Mammalian Tet-On Expression Cell Line
- Immunohistochemical Detection of Cytokines in Human Tissue Sections
- Detection of Cytokine- and Chemokine-Expressing Cells at the Single Cell Level
- Detection of Cytokine Signal Transduction “Cross-Talk” in Leukocyte Activation
- Assay System for the Effect of Prostaglandin E2 in the Determination of Polarized Cytokine Production.