Cytokine Protocols

Because cytokines regulate many different bodily functions in living organisms, mainly at the level of cell-cell communications, there is great interest in understanding their mode of action. In Cytokine Protocols, established researchers, physicians, and clinicians present their best biochemical, c...

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書目詳細資料
企業作者: SpringerLink (Online service)
其他作者: De Ley, Marc. (Editor, http://id.loc.gov/vocabulary/relators/edt)
格式: 電子 電子書
語言:English
出版: Totowa, NJ : Humana Press : Imprint: Humana, 2004.
版:1st ed. 2004.
叢編:Methods in Molecular Biology, 249
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在線閱讀:https://doi.org/10.1385/1592596673
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書本目錄:
  • Large-Scale Generation of Plasmids that Express Type I Interferon
  • Identification of trans-Acting Factors by Electrophoretic Mobility Shift Assay
  • Modulation of the Interferon-? Signal by Transfection of Cells with an Antisense-RNA Expressing Vector
  • Competitive RT-PCR to Quantify Small Amounts of mRNA
  • In SituHybridization for Cytokines in Human Tissue Biopsies
  • Purification, Identification, and Synthesis of Chemokines
  • Receptor Isolation and Characterization
  • Crystallization of Cytokine-Receptor Complexes
  • Biosensor Analysis of Receptor-Ligand Interactions
  • Analysis of SH2 Ligands and Identification of Sites of Interaction
  • Assays for Antiviral Activity
  • Assays for Cytotoxicity
  • Assays for Chemotaxis
  • In Vitro and In Vivo Assays for High-Molecular-Weight Pegylated Muteins of Granulocyte Colony-Stimulating Factor
  • Development of a Mammalian Tet-On Expression Cell Line
  • Immunohistochemical Detection of Cytokines in Human Tissue Sections
  • Detection of Cytokine- and Chemokine-Expressing Cells at the Single Cell Level
  • Detection of Cytokine Signal Transduction “Cross-Talk” in Leukocyte Activation
  • Assay System for the Effect of Prostaglandin E2 in the Determination of Polarized Cytokine Production.