Vaccinia Virus and Poxvirology Methods and Protocols /

Recombinant vaccinia viruses have become important in recent years, both as protein expression vectors, and-in the age of bioterrorism-as powerful tools for developing new smallpox vaccines, diagnostics, and understanding of poxvirus pathogenesis. In Vaccinia Virus and Poxvirology: Methods and Proto...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
مؤلف مشترك: SpringerLink (Online service)
مؤلفون آخرون: Isaacs, Stuart N. (المحرر, http://id.loc.gov/vocabulary/relators/edt)
التنسيق: الكتروني كتاب الكتروني
اللغة:English
منشور في: Totowa, NJ : Humana Press : Imprint: Humana, 2004.
الطبعة:1st ed. 2004.
سلاسل:Methods in Molecular Biology, 269
الموضوعات:
الوصول للمادة أونلاين:https://doi.org/10.1385/1592597890
الوسوم: إضافة وسم
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جدول المحتويات:
  • Working Safely with Vaccinia Virus
  • Construction and Isolation of Recombinant Vaccinia Virus Using Genetic Markers
  • Construction of Recombinant Vaccinia Virus
  • Transient and Inducible Expression of Vaccinia/T7 Recombinant Viruses
  • Construction of Recombinant Vaccinia Viruses Using Leporipoxvirus-Catalyzed Recombination and Reactivation of Orthopoxvirus DNA
  • Construction of cDNA Libraries in Vaccinia Virus
  • Construction and Isolation of Recombinant MVA
  • Growing Poxviruses and Determining Virus Titer
  • Rapid Preparation of Vaccinia Virus DNA Template for Analysis and Cloning by PCR
  • Orthopoxvirus Diagnostics
  • An In Vitro Transcription System for Studying Vaccinia Virus Early Genes
  • An In Vitro Transcription System for Studying Vaccinia Virus Late Genes
  • Studying Vaccinia Virus RNA Processing In Vitro
  • Methods for Analysis of Poxvirus DNA Replication
  • Studying the Binding and Entry of the Intracellular and Extracellular Enveloped Forms of Vaccinia Virus
  • Pox, Dyes, and Videotape
  • Interaction Analysis of Viral Cytokine-Binding Proteins Using Surface Plasmon Resonance
  • Monitoring of Human Immunological Responses to Vaccinia Virus
  • Vaccinia Virus as a Tool for Immunologic Studies
  • Mouse Models for Studying Orthopoxvirus Respiratory Infections
  • Viral Glycoprotein-Mediated Cell Fusion Assays Using Vaccinia Virus Vectors
  • Use of Dual Recombinant Vaccinia Virus Vectors to Assay Viral Glycoprotein-Mediated Fusion with Transfection-Resistant Primary Cell Targets
  • Poxvirus Bioinformatics
  • Preparation and Use of Molluscum Contagiosum Virus from Human Tissue Biopsy Specimens.