Protein NMR Techniques
Carrying on in the tradition of its much praised earlier edition, A. Kristina Downing has selected new protocols describing the many recent advances in NMR methodology that are revolutionizing the field. Described in step-by-step detail by established hands-on experts, these readily reproducible tec...
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| Formato: | Electrónico eBook |
| Idioma: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2004.
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| Edición: | 2nd ed. 2004. |
| Series: | Methods in Molecular Biology,
278 |
| Subjects: | |
| Acceso en liña: | https://doi.org/10.1385/1592598099 |
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Table of Contents:
- Screening and Optimizing Protein Production in E. coli
- Isotopic Labeling of Recombinant Proteins from the Methylotrophic Yeast Pichia pastoris
- Perdeuteration/Site-Specific Protonation Approaches for High-Molecular-Weight Proteins
- Segmental Isotopic Labeling for Structural Biological Applications of NMR
- TROSY-Based Correlation and NOE Spectroscopy for NMR Structural Studies of Large Proteins
- Media for Studies of Partially Aligned States
- Residual Dipolar Couplings in Protein Structure Determination
- Projection Angle Restraints for Studying Structure and Dynamics of Biomolecules
- Characterizing Domain Interfaces by NMR
- Characterization of the Overall Rotational Diffusion of a Protein From 15N Relaxation Measurements and Hydrodynamic Calculations
- TROSY-Based NMR Experiments for the Study of Macromolecular Dynamics and Hydrogen Bonding
- Measurement of Intermediate Exchange Phenomena
- NMR Studies of Partially Folded Molten-Globule States
- Structure Determination of Protein Complexes by NMR
- NMR Studies of Protein-Nucleic Acid Interactions
- Using NMRView to Visualize and Analyze the NMR Spectra of Macromolecules
- Automated NMR Structure Calculation With CYANA
- NOE Assignment With ARIA 2.0
- Membrane Protein Structure Determination Using Solid-State NMR.