Circadian Rhythms Methods and Protocols /

The aim of Circadian Rhythms is to provide a resource that can be adopted by several types of users: those who are new to circadian biology, those who are already active in the field but are interested in learning new techniques and researchers who are considering moving to a new a model system or u...

সম্পূর্ণ বিবরণ

সংরক্ষণ করুন:
গ্রন্থ-পঞ্জীর বিবরন
সংস্থা লেখক: SpringerLink (Online service)
অন্যান্য লেখক: Rosato, Ezio. (Editor, http://id.loc.gov/vocabulary/relators/edt)
বিন্যাস: বৈদ্যুতিক বৈদ্যুতিন গ্রন্থ
ভাষা:English
প্রকাশিত: Totowa, NJ : Humana Press : Imprint: Humana, 2007.
সংস্করন:1st ed. 2007.
মালা:Methods in Molecular Biology, 362
বিষয়গুলি:
অনলাইন ব্যবহার করুন:https://doi.org/10.1007/978-1-59745-257-1
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সূচিপত্রের সারণি:
  • Overviews
  • Light, Photoreceptors, and Circadian Clocks
  • Statistical Analysis of Biological Rhythm Data
  • Rhythmic Readouts
  • Rhythmic Conidiation in Neurospora crassa
  • Monitoring and Analyzing Drosophila Circadian Locomotor Activity
  • Automated Video Image Analysis of Larval Zebrafish Locomotor Rhythms
  • Locomotor Activity in Rodents
  • Analysis of Circadian Leaf Movement Rhythms in Arabidopsis thaliana
  • Detection of Rhythmic Bioluminescence From Luciferase Reporters in Cyanobacteria
  • Analysis of Rhythmic Gene Expression in Adult Drosophila Using the Firefly Luciferase Reporter Gene
  • Monitoring Circadian Rhythms in Arabidopsis thaliana Using Luciferase Reporter Genes
  • Identification of Clock Genes
  • Specialized Techniques for Site-Directed Mutagenesis in Cyanobacteria
  • Novel Strategies for the Identification of Clock Genes Neurospora With Insertional Mutagenesis
  • Mutagenesis With Drosophila
  • Mutagenesis in Arabidopsis
  • Yeast Two-Hybrid Screening
  • Microarrays
  • Microarrays
  • Identification of Clock Genes Using Difference Gel Electrophoresis
  • Gene Expression: RNA
  • Isolation of Total RNA From Neurospora Mycelium
  • RNA Extraction From Drosophila Heads
  • Extraction of Plant RNA
  • RNA Extraction From Mammalian Tissues
  • Northern Analysis of Sense and Antisense frequency RNA in Neurospora crassa
  • RNase Protection Assay
  • Quantitative Polymerase Chain Reaction
  • Gene Expression: Proteins
  • Protein Extraction, Fractionation, and Purification From Cyanobacteria
  • Protein Extraction From Drosophila Heads
  • Plant Protein Extraction
  • Protein Extraction From Mammalian Tissues
  • Western Blotting
  • Coimmunoprecipitation Assay
  • In Vitro Phosphorylation and Kinase Assays in Neurospora crassa
  • In Vitro Systems
  • Basic Protocols for Drosophila S2 Cell Line
  • Coimmunoprecipitation on Drosophila Cells in Culture
  • Basic Protocols for Zebrafish Cell Lines
  • Manipulation of Mammalian Cell Lines for Circadian Studies
  • Reporter Assays
  • Use of Firefly Luciferase Activity Assays to Monitor Circadian Molecular Rhythms In Vivo and In Vitro
  • Suprachiasmatic Nucleus Cultures That Maintain Rhythmic Properties In Vitro
  • Microscopy Analysis
  • RNA In Situ Hybridizations on Drosophila Whole Mounts
  • In Situ Hybridization of Suprachiasmatic Nucleus Slices
  • Immunohistochemistry in Drosophila
  • Immunocytochemistry on Suprachiasmatic Nucleus Slices
  • Immunofluorescence Analysis of Circadian Protein Dynamics in Cultured Mammalian Cells.