Circadian Rhythms Methods and Protocols /
The aim of Circadian Rhythms is to provide a resource that can be adopted by several types of users: those who are new to circadian biology, those who are already active in the field but are interested in learning new techniques and researchers who are considering moving to a new a model system or u...
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| Outros Autores: | |
| Formato: | Recurso Electrónico livro electrónico |
| Idioma: | English |
| Publicado em: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2007.
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| Edição: | 1st ed. 2007. |
| Colecção: | Methods in Molecular Biology,
362 |
| Assuntos: | |
| Acesso em linha: | https://doi.org/10.1007/978-1-59745-257-1 |
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Sumário:
- Overviews
- Light, Photoreceptors, and Circadian Clocks
- Statistical Analysis of Biological Rhythm Data
- Rhythmic Readouts
- Rhythmic Conidiation in Neurospora crassa
- Monitoring and Analyzing Drosophila Circadian Locomotor Activity
- Automated Video Image Analysis of Larval Zebrafish Locomotor Rhythms
- Locomotor Activity in Rodents
- Analysis of Circadian Leaf Movement Rhythms in Arabidopsis thaliana
- Detection of Rhythmic Bioluminescence From Luciferase Reporters in Cyanobacteria
- Analysis of Rhythmic Gene Expression in Adult Drosophila Using the Firefly Luciferase Reporter Gene
- Monitoring Circadian Rhythms in Arabidopsis thaliana Using Luciferase Reporter Genes
- Identification of Clock Genes
- Specialized Techniques for Site-Directed Mutagenesis in Cyanobacteria
- Novel Strategies for the Identification of Clock Genes Neurospora With Insertional Mutagenesis
- Mutagenesis With Drosophila
- Mutagenesis in Arabidopsis
- Yeast Two-Hybrid Screening
- Microarrays
- Microarrays
- Identification of Clock Genes Using Difference Gel Electrophoresis
- Gene Expression: RNA
- Isolation of Total RNA From Neurospora Mycelium
- RNA Extraction From Drosophila Heads
- Extraction of Plant RNA
- RNA Extraction From Mammalian Tissues
- Northern Analysis of Sense and Antisense frequency RNA in Neurospora crassa
- RNase Protection Assay
- Quantitative Polymerase Chain Reaction
- Gene Expression: Proteins
- Protein Extraction, Fractionation, and Purification From Cyanobacteria
- Protein Extraction From Drosophila Heads
- Plant Protein Extraction
- Protein Extraction From Mammalian Tissues
- Western Blotting
- Coimmunoprecipitation Assay
- In Vitro Phosphorylation and Kinase Assays in Neurospora crassa
- In Vitro Systems
- Basic Protocols for Drosophila S2 Cell Line
- Coimmunoprecipitation on Drosophila Cells in Culture
- Basic Protocols for Zebrafish Cell Lines
- Manipulation of Mammalian Cell Lines for Circadian Studies
- Reporter Assays
- Use of Firefly Luciferase Activity Assays to Monitor Circadian Molecular Rhythms In Vivo and In Vitro
- Suprachiasmatic Nucleus Cultures That Maintain Rhythmic Properties In Vitro
- Microscopy Analysis
- RNA In Situ Hybridizations on Drosophila Whole Mounts
- In Situ Hybridization of Suprachiasmatic Nucleus Slices
- Immunohistochemistry in Drosophila
- Immunocytochemistry on Suprachiasmatic Nucleus Slices
- Immunofluorescence Analysis of Circadian Protein Dynamics in Cultured Mammalian Cells.