TY  - GEN
TY  - GEN
T1  - Microbial Gene Essentiality: Protocols and Bioinformatics
T2  - Methods in Molecular Biology,
A2  - Osterman, Andrei L.
A2  - Osterman, Andrei L.
A2  - Gerdes, Svetlana Y.
A2  - Gerdes, Svetlana Y.
LA  - English
PP  - Totowa, NJ
PB  - Humana Press : Imprint: Humana
YR  - 2008
ED  - 1st ed. 2008.
UL  - http://discoverylib.upm.edu.my/discovery/Record/978-1-59745-321-9
AB  - Microbial Gene Essentiality: Protocols and Bioinformatics contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. In addition to wet-lab protocols, the book describes (i) statistical methods essential for planning successful large-scale essentiality screens, as well for data evaluation and analysis; (ii) in-silico prediction of gene essentiality using genome-scale reconstructed metabolic models; and (iii) data integration and comparative analysis in the context of genomic databases. This volume provides researchers with a first-stop guide for choosing the most appropriate strategy for planned essentiality studies. Experimental and computational aspects are equally important in genome-scale gene essentiality analysis, as in all other genomic technologies, and Microbial Gene Essentiality: Protocols and Bioinformatics reflects both of these aspects. All protocols follow the successful Methods in Molecular Biology™ series format, each offering an introduction outlining the principles behind the techniques, step-by-step instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls, and are intended for both novice and expert scientists.
OP  - 486
CN  - QH345
SN  - 9781597453219
KW  - Biochemistry.
KW  - Cell biology.
KW  - Human genetics.
KW  - Biochemistry, general.
KW  - Cell Biology.
KW  - Human Genetics.
ER  -