TY  - GEN
TY  - GEN
T1  - RT-PCR Protocols Second Edition
T2  - Methods in Molecular Biology,
A2  - King, Nicola.
A2  - King, Nicola.
LA  - English
PP  - Totowa, NJ
PB  - Humana Press : Imprint: Humana
YR  - 2010
ED  - 2nd ed. 2010.
UL  - http://discoverylib.upm.edu.my/discovery/Record/978-1-60761-629-0
AB  - Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.
OP  - 341
CN  - RB155-155.8
SN  - 9781607616290
KW  - Human genetics.
KW  - Gene expression.
KW  - Human Genetics.
KW  - Gene Expression.
ER  -