Nitrogen Fixation Methods and Protocols /
Few problems in protein biochemistry have proven to be as challenging and recalcitrant as the molecular description of nitrogenase, the catalyst of one of the most remarkable chemical transformations in biological systems: the nucleotide-dependent reduction of atmospheric dinitrogen to bioavailable...
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| Formato: | Recurso Electrónico livro electrónico |
| Idioma: | English |
| Publicado em: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2011.
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| Edição: | 1st ed. 2011. |
| Colecção: | Methods in Molecular Biology,
766 |
| Assuntos: | |
| Acesso em linha: | https://doi.org/10.1007/978-1-61779-194-9 |
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Sumário:
- Historic Overview of Nitrogenase Research
- Mechanism of Mo-Dependent Nitrogenase
- Assembly of Nitrogenase MoFe Protein
- Genomic Analysis of Nitrogen Fixation
- Enzymatic Systems with Homology to Nitrogenase
- Molecular Biology and Genetic Engineering in Nitrogen Fixation
- Purification of Nitrogenase Proteins
- Assays of Nitrogenase Reaction Products
- Methods for Nitrogenase-Like Dark Operative Protochlorophyllide Oxidoreductase
- X-Ray Crystallography
- X-Ray Absorption Spectroscopy
- Small Angle X-Ray Scattering Spectroscopy
- Electron Paramagnetic Resonance Spectroscopy
- Magnetic Circular Dichroism Spectroscopy
- Mössbauer Spectroscopy
- Protocols for Cofactor Isoation of Nitrogenase
- Techniques for Functional and Structural Modeling of Nitrogenase
- Nitrogenase Structure and Function Relationships by Density Functional Theory
- Modeling the MoFe Nitrogenase System with Broken Symmetry Density Functional Theory.