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Ribosome Display and Related Technologies Methods and Protocols /

Display technologies have become a very powerful way of generating therapeutic lead molecules and specific reagents for increasing our understanding of biology; however, despite being first described shortly after phage display, the use of ribosome display and related methods have been much less wid...

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Bibliografiske detaljer
Institution som forfatter: SpringerLink (Online service)
Andre forfattere: Douthwaite, Julie A. (Editor, http://id.loc.gov/vocabulary/relators/edt), Jackson, Ronald H. (Editor, http://id.loc.gov/vocabulary/relators/edt)
Format: Electronisk eBog
Sprog:English
Udgivet: Totowa, NJ : Humana Press : Imprint: Humana, 2012.
Udgivelse:1st ed. 2012.
Serier:Methods in Molecular Biology, 805
Fag:
Proteins .
Protein Science.
Online adgang:https://doi.org/10.1007/978-1-61779-379-0
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Indholdsfortegnelse:
  • Ribosome Display: A Perspective
  • Preparation and Testing of E.coli S30 In Vitro Transcription Translation Extracts
  • Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate
  • Stabilized Ribosome Display for In Vitro Selection
  • Eukaryotic Ribosome Display with In Situ DNA Recovery
  • mRNA Display Using Covalent Coupling of mRNA to Translated Proteins
  • SNAP Display: In Vitro Protein Evolution in Microdroplets
  • cDNA Display: Rapid Stabilization of mRNA Display
  • Optimization of Antibody Affinity by Ribosome Display Using Error-Prone or Site-Directed Mutagenesis
  • Affinity Maturation of Phage Display Antibody Populations using Ribosome Display
  • Evolution of Protein Stability Using Ribosome Display
  • Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries
  • Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display
  • Peptide Screening Using PURE Ribosome Display
  • Rapid Selection of High Affinity Binders Using Ribosome Display
  • mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries
  • Identification of Candidate Vaccine Genes Using Ribosome Display
  • Ribosome Display for the Selection of Sac7d Scaffolds
  • Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers
  • Update on Pure Translation Display with Unnatural Amino Acid Incorporation
  • In Vitro Selection of Unnatural Cyclic Peptide Libraries Via mRNA Display
  • Optimization of CAT-354, a Therapeutic Antibody Directed Against Interleukin-13, Using Ribosome Display
  • Affinity Maturation and Functional Dissection of a Humanized Anti-RAGE Monoclonal Antibody by Ribosome Display.

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