Ribosome Display and Related Technologies Methods and Protocols /
Display technologies have become a very powerful way of generating therapeutic lead molecules and specific reagents for increasing our understanding of biology; however, despite being first described shortly after phage display, the use of ribosome display and related methods have been much less wid...
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| Outros autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2012.
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| Edición: | 1st ed. 2012. |
| Series: | Methods in Molecular Biology,
805 |
| Subjects: | |
| Acceso en liña: | https://doi.org/10.1007/978-1-61779-379-0 |
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Table of Contents:
- Ribosome Display: A Perspective
- Preparation and Testing of E.coli S30 In Vitro Transcription Translation Extracts
- Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate
- Stabilized Ribosome Display for In Vitro Selection
- Eukaryotic Ribosome Display with In Situ DNA Recovery
- mRNA Display Using Covalent Coupling of mRNA to Translated Proteins
- SNAP Display: In Vitro Protein Evolution in Microdroplets
- cDNA Display: Rapid Stabilization of mRNA Display
- Optimization of Antibody Affinity by Ribosome Display Using Error-Prone or Site-Directed Mutagenesis
- Affinity Maturation of Phage Display Antibody Populations using Ribosome Display
- Evolution of Protein Stability Using Ribosome Display
- Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries
- Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display
- Peptide Screening Using PURE Ribosome Display
- Rapid Selection of High Affinity Binders Using Ribosome Display
- mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries
- Identification of Candidate Vaccine Genes Using Ribosome Display
- Ribosome Display for the Selection of Sac7d Scaffolds
- Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers
- Update on Pure Translation Display with Unnatural Amino Acid Incorporation
- In Vitro Selection of Unnatural Cyclic Peptide Libraries Via mRNA Display
- Optimization of CAT-354, a Therapeutic Antibody Directed Against Interleukin-13, Using Ribosome Display
- Affinity Maturation and Functional Dissection of a Humanized Anti-RAGE Monoclonal Antibody by Ribosome Display.