Next-Generation MicroRNA Expression Profiling Technology Methods and Protocols /

The rapid pace of microRNA (miRNA) research continues to drive the advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed during the past few yea...

Mô tả đầy đủ

Đã lưu trong:
Chi tiết về thư mục
Tác giả của công ty: SpringerLink (Online service)
Tác giả khác: Fan, Jian-Bing. (Biên tập viên, http://id.loc.gov/vocabulary/relators/edt)
Định dạng: Điện tử eBook
Ngôn ngữ:English
Được phát hành: Totowa, NJ : Humana Press : Imprint: Humana, 2012.
Phiên bản:1st ed. 2012.
Loạt:Methods in Molecular Biology, 822
Những chủ đề:
Truy cập trực tuyến:https://doi.org/10.1007/978-1-61779-427-8
Các nhãn: Thêm thẻ
Không có thẻ, Là người đầu tiên thẻ bản ghi này!
Mục lục:
  • Controlling miRNA Regulation in Disease
  • Introduction to miRNA Profiling Technologies and Cross-Platform Comparison
  • Stem-Loop RT-qPCR for MicroRNA Expression Profiling
  • Poly(T) Adaptor RT-PCR
  • MicroRNA In Situ Hybridization
  • Agilent MicroRNA Microarray Profiling System
  • miRNA Gene Expression Profiling Using Illumina Universal BeadChips
  • MicroRNA Expression Analysis Using the Affymetrix Platform
  • Individualized miRNA Assay Panels Using Optically Encoded Beads
  • Microfluidic Primer Extension Assay (MPEA)
  • MicroRNA Profiling Using µParaflo Microfluidic Array Technology
  • MicroRNA Expression Analysis Using the Illumina MicroRNA-Seq Platform
  • Next Generation Sequencing of miRNAs with Roche 454 GS-FLX Technology: Steps for a Successful Application
  • Methods for Small RNA Preparation for Digital Gene Expression Profiling by Next-Generation Sequencing
  • Profiling of Short RNAs Using Helicos Single-Molecule Sequencing
  • deepBase: Annotation and Discovery of microRNAs and Other Non-Coding RNAs from Deep Sequencing Data
  • PhenomiR: MicroRNAs in Human Diseases and Biological Processes
  • miRNA Expression Profiling: From Reference Genes to Global Mean Normalization
  • miRNA Data Analysis: Next-Gen Sequencing
  • Integrated miRNA Expression Analysis and Target Prediction
  • miRNAs in Human Cancer
  • Blood-Based miRNA Preparation for Non-Invasive Biomarker Development.