TY - GEN TY - GEN T1 - Single Domain Antibodies Methods and Protocols T2 - Methods in Molecular Biology, A2 - Saerens, Dirk. A2 - Saerens, Dirk. A2 - Muyldermans, Serge. A2 - Muyldermans, Serge. LA - English PP - Totowa, NJ PB - Humana Press : Imprint: Humana YR - 2012 ED - 1st ed. 2012. UL - http://discoverylib.upm.edu.my/discovery/Record/978-1-61779-968-6 AB - The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications.  In Single Domain Antibodies: Methods and Protocols, expert researchers examine single variable domain antibody fragments, referred to as VH, VL, VHH or VNAR. These fragments are the smallest intact antigen-binding fragments that can be produced recombinantly at low cost.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. OP - 580 CN - QR180-189.5 SN - 9781617799686 KW - Immunology. KW - Antibodies. ER -