Peroxisome Proliferator-Activated Receptors (PPARs) Methods and Protocols /

All three peroxisome proliferator-activated receptor (PPAR) subtypes share a high degree of structural homology but differ in function, tissue distribution and ligand specificity. PPARs play critical roles as regulators of numerous physiological as well as pathophysiological pathways, and efforts ar...

সম্পূর্ণ বিবরণ

সংরক্ষণ করুন:
গ্রন্থ-পঞ্জীর বিবরন
সংস্থা লেখক: SpringerLink (Online service)
অন্যান্য লেখক: Badr, Mostafa Z. (Editor, http://id.loc.gov/vocabulary/relators/edt), Youssef, Jihan A. (Editor, http://id.loc.gov/vocabulary/relators/edt)
বিন্যাস: বৈদ্যুতিক বৈদ্যুতিন গ্রন্থ
ভাষা:English
প্রকাশিত: Totowa, NJ : Humana Press : Imprint: Humana, 2013.
সংস্করন:1st ed. 2013.
মালা:Methods in Molecular Biology, 952
বিষয়গুলি:
অনলাইন ব্যবহার করুন:https://doi.org/10.1007/978-1-62703-155-4
ট্যাগগুলো: ট্যাগ যুক্ত করুন
কোনো ট্যাগ নেই, প্রথমজন হিসাবে ট্যাগ করুন!
সূচিপত্রের সারণি:
  • PPARs: History and Advances
  • PPAR-Alpha Cloning, Expression and Characterization
  • PPARGC1A and PPARG Genotyping. Beginner’s Guide to Genotyping with Unlabeled Probes
  • Generation of an Inducible, Cardiomyocyte-Specific Transgenic Mouse Model with PPARβ/d Overexpression
  • Specific knockdown of PPARd Gene in Colon Cancer Cells by Lentivirus-Mediated RNA Interfering
  • Dominant-Negative and Knockdown Approaches to Studying PPAR activity
  • Producing PPARgamma2 Knockdown in Mouse Liver
  • Adipose Tissue-Specific PPARg Gene Targeting
  • Site-Directed Mutagenesis to Study the Role of Specific Amino Acids in the Ligand Binding Domain of PPARs
  • PPAR SUMOylation: Some Useful Experimental Tips
  • Analyzing Phosphorylation-Dependent Regulation of Subcellular Transcriptional Activity of Transcriptional Coactivator NT-PGC-1a
  • In vivo Studies of PPAR-Chromatin Interactions: Chromatin Immunoprecipitation for Single-Locus and Genomewide Analyses
  • FISH Analysis Using PPARg  Specific Probes for Detection of PAX8-PPARg Translocation in Follicular Thyroid Neoplasms
  • Immunohistochemical Techniques to Identify and Localize Proteins of Interest in Paraffin-Embedded Tissue Sections
  • Determination of PPAR Expression by Western-Blot
  • Fluorescence Resonance Energy Transfer Techniques to Study Ligand-Mediated Interactions of PPARs with Coregulators
  • Estimation of the PPARa Agonism of Fibrates by a Combined MM-Docking Approach
  • Combined Biophysical and Cell-based Approaches for the Assessment of Ligand Binding to PPARg
  • Exploring PPAR Modulation in Experimental Mice
  • Induction of Adipogenic Differentiation in Three-Dimensional Culture Model on a Novel Microfabricated Scaffold
  • Analyzing PPAa/Ligand Interactions by Chemical Cross-Linking and High-Resolution Mass Spectrometry
  • Synthesis, Mass Spectrometric Characterization, and Analysis of the PPARd Agonist GW1516 and its Major Human Metabolites – Targets in Sports Drug Testing
  • LC-MS-Based Method for the Qualitative and Quantitative Analysis of the Novel PPARg Agonist KR-62980
  • Behavioral Paradigms to Evaluate PPAR Modulation in Animal Models of Brain Injury.