TY  - GEN
TY  - GEN
T1  - Enzyme Engineering Methods and Protocols
T2  - Methods in Molecular Biology,
A2  - Samuelson, James C.
A2  - Samuelson, James C.
LA  - English
PP  - Totowa, NJ
PB  - Humana Press : Imprint: Humana
YR  - 2013
ED  - 1st ed. 2013.
UL  - http://discoverylib.upm.edu.my/discovery/Record/978-1-62703-293-3
AB  - Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes or screening strategies. Enzyme Engineering: Methods and Protocols provides guidance to investigators wishing to create enzyme variants with desired properties. This detailed volume covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes. It also highlights the engineering of two difference types of rare-cutting endonucleases that show great potential in gene therapy applications: The newest development is the emergence of TAL effector nucleases or TALENs. Chapters describe newly developed technologies in sufficient detail so that each method can be practiced in a standard molecular biology laboratory. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible Enzyme Engineering: Methods and Protocols will be valuable for scientists with a budding interest in protein engineering as well as veterans looking for new approaches to apply in established discovery programs.
OP  - 252
CN  - QH345
SN  - 9781627032933
KW  - Biochemistry.
KW  - Enzymology.
KW  - Biochemistry, general.
ER  -