Cyclic Nucleotide Signaling in Plants Methods and Protocols /

Over the last two decades there has been a growing interest in cyclic nucleotide research in plants with an emphasis on the elucidation of the roles of cGMP and cAMP. In Cyclic Nucleotide Signaling in Plants: Methods and Protocols, expert researchers in the field detail many approaches to better und...

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Bibliografiska uppgifter
Institutionell upphovsman: SpringerLink (Online service)
Övriga upphovsmän: Gehring, Chris. (Utgivare, redaktör, sammanställare, http://id.loc.gov/vocabulary/relators/edt)
Materialtyp: Elektronisk E-bok
Språk:English
Publicerad: Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Upplaga:1st ed. 2013.
Serie:Methods in Molecular Biology, 1016
Ämnen:
Länkar:https://doi.org/10.1007/978-1-62703-441-8
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Innehållsförteckning:
  • Molecular Methods for the Study of Signal Transduction in Plants
  • Recombinant Expression and Functional Testing of Candidate Adenylate Cyclase Domains
  • Quantification of Cyclic Dinucleotides by Reversed-Phase LC-MS/MS
  • Determination of ADP-ribosyl cyclase activity, cyclic ADP-ribose and Nicotinamide adenine dinucleotide phosphate in Tissue Extracts
  • In vivo imaging of cGMP in plants
  • Characterization of Heterologously Expressed Transporter Genes by Patch- and Voltage-Clamp Methods: Application to Cyclic Nucleotide-Dependent Responses
  • Non-invasive Microelectrode Ion Flux Estimation Technique (MIFE) for the study of the regulation of root membrane transport by cyclic nucleotides
  • Calcium imaging of the cyclic nucleotide response
  • Identification and Quantification of Signal Molecule-dependent Protein Phosphorylation
  • Comparative Gel-based (Phospho)proteomics in Response to Signaling Molecules
  • An Affinity Pull-down Approach to Identify the Plant Cyclic Nucleotide Interactome
  • Structural and Functional Characterization of Receptor Kinases with Nucleotide Cyclase Activit
  • Computational Identification of Candidate Nucleotide Cyclases in Higher Plants
  • Identification of Cyclic Nucleotide Gated Channels Using Regular Expressions
  • Inferring biological functions of guanylyl cyclases with computational methods
  • Identification and Characterization of Cyclic Nucleotide Phosphodiesterases
  • Detection of reactive oxygen species down-stream of cyclic nucleotide signals in plants
  • Measurement of nitric oxide in plant tissue using difluorofluorescein and oxyhaemoglobin
  • Infra-red Gas Analysis (IRGA) Technique for the Study of the Regulation of Photosynthetic Responses.