Legume Genomics Methods and Protocols /

Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and...

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Ente Autore: SpringerLink (Online service)
Altri autori: Rose, Ray J. (Redattore, http://id.loc.gov/vocabulary/relators/edt)
Natura: Elettronico eBook
Lingua:English
Pubblicazione: Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Edizione:1st ed. 2013.
Serie:Methods in Molecular Biology, 1069
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Accesso online:https://doi.org/10.1007/978-1-62703-613-9
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Sommario:
  • The Model Legume Genomes
  • Fluorescent In Situ Hybridization (FISH) on Pachytene Chromosomes as a Tool for Genome Characterization
  • Targeted Mutagenesis for Functional Analysis of Gene Duplication in Legumes
  • RNA-Seq for Transcriptome Analysis in Non-Model Plants
  • Functional Analysis of Legume Genome Arrays
  • Genome-Wide Identification of MicroRNAs in Medicago truncatula by High-Throughput Sequencing
  • Determining Abundance of MicroRNAs and Other Small RNAs in Legumes
  • Forward Genetics Screening of Medicago truncatula Tnt1 Insertion Lines
  • Reverse Genetics in Medicago truncatula Using a TILLING Mutant Collection
  • High-Throughput and Targeted Genotyping of Lotus japonicus LORE1 Insertion Mutants
  • Isolation and Forward Genetic Analysis of Developmental Genes in Pea
  • Gene Silencing in Medicago truncatula Roots Using RNAi
  • Molecular Markers for Genetics and Plant Breeding: The MFLP Marker System and Its Application in Narrow-Leafed Lupin (Lupinus angustifolius)
  • Stable Transformation of Medicago truncatula cv. Jemalong for Gene Analysis Using Agrobacterium tumefaciens
  • Transient Gene Expression in Medicago truncatula Leaves Via Agroinfiltration
  • Subcellular Localization of Transiently-Expressed Fluorescent Fusion Proteins
  • Proteomics and the Analysis of Nodulation
  • Phosphoproteomic Analysis of Peptides
  • Plant Metabolomics: From Experimental Design to Knowledge Extraction.