Analyzing Microbes Manual of Molecular Biology Techniques /
This Springer Protocols manual is a practical guide to the application of key molecular biology techniques in microbiological research. The focus is on experimental protocols, which are presented in an easy-to-follow way, as step-by-step procedures for direct use in the laboratory. Notes on how to s...
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| Andre forfattere: | , , |
| Format: | Electronisk eBog |
| Sprog: | English |
| Udgivet: |
Berlin, Heidelberg :
Springer Berlin Heidelberg : Imprint: Springer,
2013.
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| Udgivelse: | 1st ed. 2013. |
| Serier: | Springer Protocols Handbooks,
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| Fag: | |
| Online adgang: | https://doi.org/10.1007/978-3-642-34410-7 |
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Indholdsfortegnelse:
- Microbial DNA extraction, purification and quantitation
- Fluorescent based detection, quantitation and expression of wviral gene by qRT-PCR
- Restriction enzymes and their role in microbiology
- Genetic fingerprinting techniques for molecular characterization of microbes
- Agarose gel electrophoresis and Polyacrylamide agarose gel electrophoresis: Methods and principles
- Molecular identification of microbes
- Preservation and maintenance of microbial cultures
- Microbes from extreme environment: Molecular identification procedures
- ELISA based identification and detection of microbes
- Analysis of microbial diversity and construction of metagenomic library
- Bioinformatics tools for interpretation of data used in molecular identification
- Molecular phylogenetics of microbes
- Microarray technology: Basic concept, protocols and applications
- Microarray analysis of different functional genes of microorganisms
- DNA cloning and sequencing
- Biological sequence analysis: Algorithms and statistical methods.