Analyzing Microbes Manual of Molecular Biology Techniques /

This Springer Protocols manual is a practical guide to the application of key molecular biology techniques in microbiological research. The focus is on experimental protocols, which are presented in an easy-to-follow way, as step-by-step procedures for direct use in the laboratory. Notes on how to s...

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Ente Autore: SpringerLink (Online service)
Altri autori: Arora, Dilip Kumar. (Redattore, http://id.loc.gov/vocabulary/relators/edt), Das, Surajit. (Redattore, http://id.loc.gov/vocabulary/relators/edt), Sukumar, Mesapogu. (Redattore, http://id.loc.gov/vocabulary/relators/edt)
Natura: Elettronico eBook
Lingua:English
Pubblicazione: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 2013.
Edizione:1st ed. 2013.
Serie:Springer Protocols Handbooks,
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Accesso online:https://doi.org/10.1007/978-3-642-34410-7
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Sommario:
  • Microbial DNA extraction, purification and quantitation
  • Fluorescent based detection, quantitation and expression of wviral gene by qRT-PCR
  • Restriction enzymes and their role in microbiology
  • Genetic fingerprinting techniques  for molecular characterization of microbes
  • Agarose gel electrophoresis and Polyacrylamide agarose gel electrophoresis: Methods and principles
  • Molecular identification of microbes
  • Preservation and maintenance of microbial cultures
  • Microbes from extreme environment: Molecular identification procedures
  • ELISA based identification and detection of microbes
  • Analysis of microbial diversity and construction of metagenomic library
  • Bioinformatics tools for interpretation of data used in molecular identification
  • Molecular phylogenetics of microbes
  • Microarray technology: Basic concept, protocols and applications
  • Microarray analysis of different functional genes of microorganisms
  • DNA cloning and sequencing
  • Biological sequence analysis: Algorithms and statistical methods.