Characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in Malaysia
Coagulase-negative staphylococci (CoNS) are often considered as contaminants or normal skin flora but in the past few decades, they had emerged as pathogenic bacteria causing serious infections especially in patients with prosthetic medical devices. Their ability to produce biofilm on polymer sur...
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oai:ethesis.upm.edu.my:13417 http://ethesis.upm.edu.my/id/eprint/13417/ Characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in Malaysia Vnayakam, Subashini Coagulase-negative staphylococci (CoNS) are often considered as contaminants or normal skin flora but in the past few decades, they had emerged as pathogenic bacteria causing serious infections especially in patients with prosthetic medical devices. Their ability to produce biofilm on polymer surfaces results in treatment failure as it increases the resistance to antibiotics and host defense. Biofilms formation are mediated by polysaccharide intercellular adhesin (PIA), encoded by intercellular adhesion (ica) genes and it has been postulated that the presence of ica genes in CoNS is associated with antimicrobial resistance and responsible for catheter and medical device-related sepsis. The aim of this study is to determine the species distribution, antibiotic susceptibility pattern and to detect the presence of ica genes (icaA, icaD, icaB, and icaC) among CoNS isolated from blood culture. This cross-sectional study was conducted between January 2015 and December 2015, with CoNS isolates obtained from the blood cultures of patients at Hospital Serdang. Coagulase negative staphylococci were identified by gram staining, catalase and coagulase test, followed by species identification by Analytical Profile Index (API) Staph identification strips. Antimicrobial susceptibility testing was performed using Kirby Bauer method interpreted according to the Clinical and Laboratory Standards Institute guidelines. The presence of ica genes were detected using multiplex polymerase chain reaction. Staphylococcus epidermidis dominated the total number of species isolated (n=64, 40.0%). A total of 160 CoNS, 72.5% were resistant to penicillin and 60% were methicillin-resistant (MR) CoNS. Majority of CoNS harbored icaD (59.3%) and in 4 (2.5%) strains, all icaADBC genes were observed. Detection of ica genes indicates CoNS are able to produce biofilm that causes serious nosocomial infections and should not always be reported as colonizer or contaminants. 2017-10 Thesis NonPeerReviewed text en http://ethesis.upm.edu.my/id/eprint/13417/1/FPSK%28M%29%202018%2049%20t.pdf Vnayakam, Subashini (2017) Characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in Malaysia. Masters thesis, Universiti Putra Malaysia. (FPSK(m) 2018 49). |
| institution |
UPM eTHESES |
| collection |
UPM eTHESES |
| language |
English |
| description |
Coagulase-negative staphylococci (CoNS) are often considered as contaminants or
normal skin flora but in the past few decades, they had emerged as pathogenic bacteria
causing serious infections especially in patients with prosthetic medical devices. Their
ability to produce biofilm on polymer surfaces results in treatment failure as it increases
the resistance to antibiotics and host defense. Biofilms formation are mediated by
polysaccharide intercellular adhesin (PIA), encoded by intercellular adhesion (ica) genes
and it has been postulated that the presence of ica genes in CoNS is associated with
antimicrobial resistance and responsible for catheter and medical device-related sepsis.
The aim of this study is to determine the species distribution, antibiotic susceptibility
pattern and to detect the presence of ica genes (icaA, icaD, icaB, and icaC) among CoNS
isolated from blood culture. This cross-sectional study was conducted between January
2015 and December 2015, with CoNS isolates obtained from the blood cultures of
patients at Hospital Serdang. Coagulase negative staphylococci were identified by gram
staining, catalase and coagulase test, followed by species identification by Analytical
Profile Index (API) Staph identification strips. Antimicrobial susceptibility testing was
performed using Kirby Bauer method interpreted according to the Clinical and
Laboratory Standards Institute guidelines. The presence of ica genes were detected using
multiplex polymerase chain reaction. Staphylococcus epidermidis dominated the total
number of species isolated (n=64, 40.0%). A total of 160 CoNS, 72.5% were resistant to
penicillin and 60% were methicillin-resistant (MR) CoNS. Majority of CoNS harbored
icaD (59.3%) and in 4 (2.5%) strains, all icaADBC genes were observed. Detection of
ica genes indicates CoNS are able to produce biofilm that causes serious nosocomial
infections and should not always be reported as colonizer or contaminants. |
| format |
Thesis |
| author |
Vnayakam, Subashini |
| spellingShingle |
Vnayakam, Subashini Characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in Malaysia |
| author_facet |
Vnayakam, Subashini |
| author_sort |
Vnayakam, Subashini |
| title |
Characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in Malaysia |
| title_short |
Characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in Malaysia |
| title_full |
Characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in Malaysia |
| title_fullStr |
Characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in Malaysia |
| title_full_unstemmed |
Characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in Malaysia |
| title_sort |
characterization and presence of ica genes in coagulase-negative staphylococci isolates in a public hospital in malaysia |
| publishDate |
2017 |
| url |
http://ethesis.upm.edu.my/id/eprint/13417/1/FPSK%28M%29%202018%2049%20t.pdf |
| _version_ |
1819311436721029120 |
| score |
13.4562235 |