TY  - GEN
T1  - Comparison of bovine embryonic development of parthenogenetically activated oocytes by different combinations of chemical treatments
A1  - Siti Khadijah, I.
LA  - English
YR  - 2016
UL  - http://discoverylib.upm.edu.my/discovery/Record/oai:http:--agris.upm.edu.my:0-10795
AB  - Oocyte activation is the efficient method or step for successful animal cloning. This study was carried out to evaluate the embryonic development for both parthenotes and fertilised embryos according to the oocyte grades (Grades A, B and C). Recovered oocytes according to the oocyte grades were divided into various combination treatments: (i) A23187+6-DMAP, (ii) Iono+6-DMAP, (iii) EtOH+6-DMAP, (iv) Iono+CHX, and (v) IVF (control). Treatment with combination of 10 μM Iono+2 mM 6-DMAP indicated the highest embryonic development rates for 8-cell, morula and blastocyst stages compared to the other combination treatments. There were significant differences between combination of Iono+6-DMAP and IVF control at morula (24.3 vs. 3.4%) and blastocyst stages (7.1 vs. 0.0%), respectively. There was no consistent relationship between oocyte grades and embryo development rates. The data also showed that no differences were observed in blastocyst rates for each oocyte grade for all activation treatments including IVF control. In conclusion, to activate bovine oocytes for later embryonic development stages (8-cell, morula and blastocyst), the best combination treatment obtained in this study was the combination of 10 μM Iono (5 min)+2 mM 6-DMAP (4 h), regardless of the grades of oocytes, including the importance of the optimal combination of chemical activation to produce bovine embryos in vitro.
KW  - Cloning
KW  - Parthenogenesis
KW  - Bovines
KW  - Oocytes
KW  - Chemical analysis (methods)
KW  - Embryonic development
ER  -