UNIMARC/MARC: Antioxidant properties and antiproliferative effect of brewers’ rice extract (temukut) on selected cancer cell lines

Antioxidant properties and antiproliferative effect of brewers’ rice extract (temukut) on selected cancer cell lines

Temukut, or brewers’ rice, is a mixture of broken rice, rice bran, and rice germ. Extensive studies have been conducted on rice bran, which possesses various health benefits. Temukut, however has been less well studied. The present study aimed to investigate the antioxidant and growth inhibition pro...

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Hlavní autoři:	Tan, B. L., Norhaizan, M. E., Suhaniza, H. J., Lai, C. C., Norazalina, S., Roselina, K.
Médium:	Journal Contribution
Jazyk:	English
Vydáno:	2016
Témata:	Antioxidants Brewers grains Cancer (disease) Growth inhibition Carotenes Bleaching Free radicals Phenolic compounds Vitamin e Phytic acid Cytotoxicity Methanol Oryza sativa High performance liquid chromatography Milling industry Drug plants Tissue proliferation Clinical examination
On-line přístup:	http://agris.upm.edu.my:8080/dspace/handle/0/10947
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spelling	oai:http:--agris.upm.edu.my:0-10947Antioxidant properties and antiproliferative effect of brewers’ rice extract (temukut) on selected cancer cell linesTan, B. L.Norhaizan, M. E.Suhaniza, H. J.Lai, C. C.Norazalina, S.Roselina, K.AntioxidantsBrewers grainsCancer (disease)Growth inhibitionCarotenesBleachingFree radicalsPhenolic compoundsVitamin ePhytic acidCytotoxicityMethanolOryza sativaHigh performance liquid chromatographyMilling industryDrug plantsTissue proliferationClinical examinationTemukut, or brewers’ rice, is a mixture of broken rice, rice bran, and rice germ. Extensive studies have been conducted on rice bran, which possesses various health benefits. Temukut, however has been less well studied. The present study aimed to investigate the antioxidant and growth inhibition properties of temukut extract using colon cancer (HT-29), ovary cancer (Caov-3), and liver cancer (HepG2) cell lines. The antioxidant activity was determined by the β-carotene bleaching assay, analysis of the DPPH radical scavenging capacity, and a FRAP assay. The total phenolic compounds, oryzanol, vitamin E, and phytic acid levels in temukut were also investigated. The antiproliferative activity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. There was a significant difference in the cytotoxicity of two types of temukut extract (water and methanol) for HT-29 and Caov-3 cells (p < 0.05) but not for HepG2 cells. The HepG2 cell line is the least sensitive to temukut, (IC50 = 55.30 μg/mL), whereas the highest sensitivity was observed in Caov-3 cells (IC50 =36.67 μg/mL). No cytotoxic effect of temukut was observed on normal cells (BalBlc3T3). Although the content of the phytochemicals studied (total phenolic compounds, vitamin E, oryzanol, and phytic acid) in temukut was lower than that in rice bran, as has been previously reported, the present study demonstrated temukut’s potential to inhibit the proliferation of HT-29, Caov-3, and HepG2 cells.2016-03-15T08:51:24Z2016-03-15T08:51:24Z2013Journal ContributionArticleNon-RefereedInternational Food Research Journal (Malaysia), 20(5), p. 2117-212422317546http://agris.upm.edu.my:8080/dspace/handle/0/10947enhttp://ifrj.upm.edu.my/20%20(05)%202013/11%20IFRJ%2020%20(05)%202013%20Norhaizan%20094.pdfhttp://www.oceandocs.org/license
institution	AGRIS
collection	AGRIS
language	English
topic	Antioxidants Brewers grains Cancer (disease) Growth inhibition Carotenes Bleaching Free radicals Phenolic compounds Vitamin e Phytic acid Cytotoxicity Methanol Oryza sativa High performance liquid chromatography Milling industry Drug plants Tissue proliferation Clinical examination
spellingShingle	Antioxidants Brewers grains Cancer (disease) Growth inhibition Carotenes Bleaching Free radicals Phenolic compounds Vitamin e Phytic acid Cytotoxicity Methanol Oryza sativa High performance liquid chromatography Milling industry Drug plants Tissue proliferation Clinical examination Tan, B. L. Norhaizan, M. E. Suhaniza, H. J. Lai, C. C. Norazalina, S. Roselina, K. Antioxidant properties and antiproliferative effect of brewers’ rice extract (temukut) on selected cancer cell lines
description	Temukut, or brewers’ rice, is a mixture of broken rice, rice bran, and rice germ. Extensive studies have been conducted on rice bran, which possesses various health benefits. Temukut, however has been less well studied. The present study aimed to investigate the antioxidant and growth inhibition properties of temukut extract using colon cancer (HT-29), ovary cancer (Caov-3), and liver cancer (HepG2) cell lines. The antioxidant activity was determined by the β-carotene bleaching assay, analysis of the DPPH radical scavenging capacity, and a FRAP assay. The total phenolic compounds, oryzanol, vitamin E, and phytic acid levels in temukut were also investigated. The antiproliferative activity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. There was a significant difference in the cytotoxicity of two types of temukut extract (water and methanol) for HT-29 and Caov-3 cells (p < 0.05) but not for HepG2 cells. The HepG2 cell line is the least sensitive to temukut, (IC50 = 55.30 μg/mL), whereas the highest sensitivity was observed in Caov-3 cells (IC50 =36.67 μg/mL). No cytotoxic effect of temukut was observed on normal cells (BalBlc3T3). Although the content of the phytochemicals studied (total phenolic compounds, vitamin E, oryzanol, and phytic acid) in temukut was lower than that in rice bran, as has been previously reported, the present study demonstrated temukut’s potential to inhibit the proliferation of HT-29, Caov-3, and HepG2 cells.
format	Journal Contribution
author	Tan, B. L. Norhaizan, M. E. Suhaniza, H. J. Lai, C. C. Norazalina, S. Roselina, K.
author_facet	Tan, B. L. Norhaizan, M. E. Suhaniza, H. J. Lai, C. C. Norazalina, S. Roselina, K.
author_sort	Tan, B. L.
title	Antioxidant properties and antiproliferative effect of brewers’ rice extract (temukut) on selected cancer cell lines
title_short	Antioxidant properties and antiproliferative effect of brewers’ rice extract (temukut) on selected cancer cell lines
title_full	Antioxidant properties and antiproliferative effect of brewers’ rice extract (temukut) on selected cancer cell lines
title_fullStr	Antioxidant properties and antiproliferative effect of brewers’ rice extract (temukut) on selected cancer cell lines
title_full_unstemmed	Antioxidant properties and antiproliferative effect of brewers’ rice extract (temukut) on selected cancer cell lines
title_sort	antioxidant properties and antiproliferative effect of brewers’ rice extract (temukut) on selected cancer cell lines
publishDate	2016
url	http://agris.upm.edu.my:8080/dspace/handle/0/10947
_version_	1819284684920586240
score	13.4562235

Antioxidant properties and antiproliferative effect of brewers’ rice extract (temukut) on selected cancer cell lines

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