TY  - GEN
T1  - An optimized sustainable medium for the direct lactic acid fermentation of  sago ( Metroxylon sagu  rottb.) starch by  Enterococcus faecium  DMF78
A1  - Kobayashi, V. B.
LA  - English
YR  - 2018
UL  - http://discoverylib.upm.edu.my/discovery/Record/oai:http:--agris.upm.edu.my:0-14630
AB  - A low-cost medium for the amylolytic lactic acid bacterium, 
Enterococcus faecium  DMF78, was developed by response surface methodology using sago starch as substrate. Six nitrogen sources, wort, whey, corn steep liquor (CSL), trub, soybean and mungbean flour were evaluated to completely eliminate or dramatically reduce the use of expensive yeast extract, beef extract, and proteose peptone in the medium. Highest lactic acid yield was achieved using a medium with 35.0% whey, 25.0% wort, 3.0% diluted CSL and 0.076% proteose peptone in the standard formulation of de Mann, Rogosa and Sharpe (MRS) medium.  Validation run of the optimized medium in the 2-L fermentor revealed slightly lower starch utilization (0.75 g/g) and low cell growth,  but  high  lactic  acid  yield  of  28.83  g/L  after  24  hours,  as  compared  to  fermentation using complete sago-MRS medium.
KW  - Metroxylon
KW  - Enterococcus faecium
KW  - Sago
KW  - Lactic acid
KW  - Fermentation
KW  - Starch
KW  - Lactic acid bacteria
KW  - Wort
KW  - Whey
KW  - Soybeans
KW  - Mung beans
KW  - Extracts
KW  - Yeast extracts
KW  - Beef
KW  - Proteoses
KW  - Philippines
ER  -