TY  - GEN
T1  - Caecal microflora composition in broilers fed sorghum based diets containing feed enzymes
A1  - Asad, S.
LA  - English
YR  - 2019
UL  - http://discoverylib.upm.edu.my/discovery/Record/oai:http:--agris.upm.edu.my:0-15704
AB  - This study was conducted to investigate whether dietary enzymes alter the caecal microbial profile of broilers fed sorghum-based diets. Four sorghum-based diets (918 g sorghum/kg diet) were prepared. One was the control diet and three had enzymes (xylanase, phytase and
protease) added. Broilers, 35-day-old, were reared (8 birds/cage) in an environmentally controlled shed and randomly allocated to replicated (n=4) assay diets and free access to feed and water all time. On day-42, birds were euthanized and caecal contents collected, pooled on a per/pen basis and frozen (-20 °C). The DNA was extracted from caecal samples using a bead-beating protocol and the V2V3 region
of the bacterial 16S rRNA gene amplified by PCR. Amplicons were separated on sequence difference using Denaturing Gradient Gel Electrophoresis (DGGE) and microbial profiles generated and compared.
The DGGE profiles, when analysed, indicated that there was approximately 80% similarity between caecal microflora in all types of the diet treatments. This suggests that there was no overall
difference between any of the profiles and therefore the addition of different types of feed enzymes in a sorghum-based diet had no impact on the overall composition of the broiler caecal microflora.
KW  - Sorghum
KW  - Broilers
KW  - Birds
KW  - Animal feeding
KW  - Animal feed crops
KW  - Dietetic foods
KW  - Diet treatment
KW  - Caecum
KW  - Veterinary laboratories
KW  - DNA
KW  - Spectrophotometers
KW  - Nucleic acids
KW  - Nutrient availability
KW  - Food safety
KW  - Food supply
ER  -