TY  - GEN
T1  - Silymarin supplementation attenuation of the lipopolysaccharide-induced metabolic changes in protein malnourished rats
A1  - Alhamdan, Adel A.
LA  - English
YR  - 2020
UL  - http://discoverylib.upm.edu.my/discovery/Record/oai:http:--agris.upm.edu.my:0-18075
AB  - Introduction: Protein malnutrition increases the sensitivity to septic shock by impairing antioxidation and immune response. Based on the potent antioxidant effects of silymarin, the putative protective role of silymarin against sepsis-induced oxidative damage in protein malnourished rats was investigated. Methods: Adult male Wistar rats were subjected to protein malnutrition via a low-protein diet (8% protein), with and without silymarin supplementation (30 mg/kg/day) for four weeks, and compared to a control group on a 18% protein diet At end of the experiment, the animals received intraperitoneal injections (i.p.) of 0.1 mg/ kg lipopolysaccharide (LPS) derived from Esdieridtia coil, and decapitation 24 h later. Albumin, C-reactive protein (CRP), total protein (TP), alkaline phosphates (AKP ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), tumor necrosis factor-α (TNF-α), interleultine-1β (IL-1β) and interleukine-6 (IL-6) levels were measured in serum. In the lungs and liver, thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) concentrations, superoxide dismutase (SOD), catalase (CAT), and glutathione-s-transferase (GST) activities were measured. Results: oxidative and inflammatory processes were significantly increased by the LPS injection and these changes were to a greater extent in the low-protein dietary group compared to control group. Silymarin supplementation in both dietary groups showed marked inhibition in these activities, although the affect was more prominent in the control group compared to the rats fed a low-protein diet. Conclusion: The study showed that silymarin protected against the impairment of antioxidation and immune response in protein malnourished rats, particularly in septic shock conditions.
KW  - Silymarin
KW  - Lipopolysaccharides
KW  - Antioxidants
KW  - Blood proteins
KW  - Blood serum
KW  - Alkaline phosphatase
KW  - Aspartate aminotransferase
KW  - Alanine aminotransferase
KW  - Catalase
KW  - Enzyme activity
KW  - Rats
KW  - Enzyme linked immunosorbent assay
KW  - Laboratory animals
KW  - Experimental infection
KW  - Flavonoids
KW  - Glutathione
KW  - Liver
KW  - Lungs
KW  - Inflammation
KW  - Interleukins
KW  - Oxidative stress
KW  - Serum albumin
KW  - Protein deficiencies
KW  - Immune response
KW  - Septic shock
KW  - Tumour necrosis factor
ER  -