Assessment of genetic diversity of Fusarium solani isolates on black pepper by inter-simple sequence repeat polymerase chain reaction
34 isolates of Fusarium solani were collected from major pepper growing areas in Sarawak and Johor. Isolations were carried by plating root sections from vines showing symptoms of yellowing disease on Fusarium media. Genomic DNA of Fusarium isolates was extracted using CTAB method. ISSR markers were...
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| Main Authors: | , , , , |
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| Format: | Proceedings Paper |
| Language: | English |
| Published: |
Malaysian Society for Microbiology
2021
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| Subjects: | |
| Online Access: | http://agris.upm.edu.my:8080/dspace/handle/0/18432 |
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| Summary: | 34 isolates of Fusarium solani were collected from major pepper growing areas in Sarawak and Johor. Isolations were carried by plating root sections from vines showing symptoms of yellowing disease on Fusarium media. Genomic DNA of Fusarium isolates was extracted using CTAB method. ISSR markers were amplified using 15 primers with di- or tri-nucleotide repeats in ISSR analysis. To study the genetic relationship, a pairwise similarity matrix was generated using Jaccard's similarity coefficient. Cluster analysis was performed to develop a dendrogram with NTSYS-pc using UPGMA. Based on the 15 primers, fragment size obtained ranged from 200 pb to 3600 pb and a total of 253 amplified bands were generated. The percentage of polymorphism was 98%. Dendrogram based on UPGMA clustering categorized the 34 F. solani isolates into two main clusters. Cluster 1 includes 33 isolates. This cluster was split into 2 sub cluster. Cluster 2 contained a unique isolate Fs-H6. This isolate showed a unique banding pattern and was distinct from other isolates. The values of similarity coefficients ranged from 0.145 for the lowest similarity coefficient between Fs-H6 and Fs-F2, to 0.848 for the greatest extent of similarity between Fs-K3 and Fs-H7. This work provided more information on the molecular evolution of F. solani isolates, which are essential for black pepper breeding program against the yellowing disease. |
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