TY  - GEN
T1  - Introduction of green-fluorescent protein (GFP) gene in Musa acuminata cv. mas (AA).
A1  - Kadir M. A.
LA  - English
PB  - Malaysian Society of Plant Physiology
YR  - 2021
UL  - http://discoverylib.upm.edu.my/discovery/Record/oai:http:--agris.upm.edu.my:0-18793
AB  - In order to introduce genes with agronomic value into banana, genetic transformation protocols have to be optimized. A PDS-1000/Helium delivery system was used to introduce the plasmid vector pGEM, which carries the GFP gene driven by the Ubi 1 promoter into the scalp and embryogenic cell suspension of banana cv. Mas. Three (3) bombardment variables that affect transformation efficiency were examined which were the gold microcarrier size of 1.0 I-lm, rupture disk pressure of 900, 1100 and 1350 psi and the target distance of 6, 9 and 12 cm. Transient GFP gene expression was assayed I to 14 days postbombardment which revealed that the highest number of transient transformants of scalp was obtained with the helium pressure of 1100 and 1350 psi along with a target distance of 9 cm and the highest number of transient transformants of embryogenic cell suspension was attained at 900 psi helium pressure with a target distance of 6 cm.
KW  - Musa (bananas)
KW  - Musa acuminata
KW  - Musaceae
KW  - Cultivars
KW  - Genetic transformation
KW  - Plasmids
KW  - Gene vectors
KW  - Suspension culture
KW  - Proteins
KW  - Gene expression
KW  - Genes
KW  - Tissue proliferation
KW  - Plant breeding
ER  -