TY - GEN T1 - Isolation of avian pneumovirus in broiler chickens in Malaysia A1 - Azri A. LA - English YR - 2021 UL - http://discoverylib.upm.edu.my/discovery/Record/oai:http:--agris.upm.edu.my:0-19854 AB - A reverse-transcription polymerase chain reaction (RT-PCR), using oligonucleotide primer sets, was set up to detect the presence of avian pneumovirus (APV) associated with swollen head syndrome in broiler chickens. Three field isolates of APV, designated as AP4751/97, J4753/97, were confirmed by virus isolation in trachea organ culture (TOC) and RT-PCR. KW - Broiler chickens KW - Poultry KW - Disease outbreak KW - Isolation KW - Polymerase chain reaction KW - PCR KW - Trachea KW - Sinuses KW - Rhinotracheitis KW - Pneumovirus KW - Diagnosis KW - Animal diseases ER -