Immobilization of lipase enzyme from Aspergillus niger by various techniques on different support materials
Three support materials; Amberlite IRC-50, Silica Gel and Kielserguhr were selected among nine carriers due to the highest activity obtained. The nine carriers that has been studied were inorganic materials (sea sand, silica gel, kielserguhr. A12O3, Zeolite), inorganic salts (CaCO3, CaSO4), ionexch...
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| Main Authors: | , , , , , , |
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| Format: | Proceedings Paper |
| Sprog: | English |
| Udgivet: |
Sarawak Biodiversity Centre
2022
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| Fag: | |
| Online adgang: | http://agris.upm.edu.my:8080/dspace/handle/0/21128 |
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| Summary: | Three support materials; Amberlite IRC-50, Silica Gel and Kielserguhr were selected among nine carriers due to the highest activity obtained. The nine carriers that has been studied were inorganic materials (sea sand, silica gel, kielserguhr. A12O3, Zeolite), inorganic salts (CaCO3, CaSO4), ionexchange resins (Amberlite IRC-50) and synthetic polymer (polypropylene). Immobilization of lipase enzyme from Aspergillus niger was carried out by simple adsorption, adsorption on ion exchange resins, combined adsorption and precipitation and pure precipitation. The suitability of the support materials and techniques for the immobilization of lipase enzyme was evaluated by determining the enzyme activity, protein loading, immobilization efficiency and Iypolytic activity of the immobilizates before and after freeze drying. Most of the immobilizates exhibited low enzyme activity during the hydrolytic reaction. Only those prepared by ionic adsorption on Amberlite IRC-50 and simple adsorption technique on Silica Gel and Kielserguhr showed good activity after freeze drying process i.e. 35.50 U/g, 21.00 U/g and 11.50 U/g, respectively. |
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