TY  - GEN
T1  - Thermostable serine protease from thermophilic Bacillus subtilis 50A
A1  - Norazila Yusoff
LA  - English
PB  - Malaysian Society for Microbiology
YR  - 2022
UL  - http://discoverylib.upm.edu.my/discovery/Record/oai:http:--agris.upm.edu.my:0-21247
AB  - An extracellular thermostable protease producer has been successfully isolated from Lojing hotspring (Kelantan, Malaysia). The isolate was identified as Bacillus subtilis by 16s rRNA analysis. Effect of temperature on the enzyme activity and stability was investigated. The crude protease displayed the maximal activity at 70°C. The thermostability profile exhibited that the protease was very stable at 50°C and the activity retained 89% of its original activity after heat treatment at 60°C for 30 min. The enzyme activity was totally inhibited by phenylmethyl sulphonyl fluoride (PMSF), suggested that the protease from B. subtilis 50a belongs to the family of serine protease. From the results, it showed that B. subtilis 50a is a good extracellular protease producer at high temperature and thus making it as promising thermostable serine protease producer forindustrial use.
KW  - Bacillus subtilis
KW  - Proteases
KW  - Bacteria
KW  - Isolation of microorganisms
KW  - Microbiological analysis
KW  - Water springs
KW  - Temperature
KW  - Heat treatment
KW  - Enzyme activity
KW  - Enzyme inhibitors
ER  -