TY  - GEN
T1  - Agrobacterium-mediated transformation of Hevea cells derived from in vitro and in vivo seedling cultures
A1  - Arokiaraj, P.
LA  - English
YR  - 2013
UL  - http://discoverylib.upm.edu.my/discovery/Record/oai:http:--agris.upm.edu.my:0-4009
AB  - Tumour tissue was produced on stems of in vitro and in vivo propagated rubber (Hevea brasiliensis) seedlings of PB 5/51, infected with a strain of Agrobacterium tumefaciens 541 (isolated by the Rubber Research Institute of Malaysia in 1971). The tumour tissues exhibited phytohormone-independent growth in tissue culture and produced octopine. In contrast, controls inoculated with distilled water on seedlings did not produce crown gall. Octopine-utilising Agrobacterium strain 541 was distinguished from non-utilisers on a solid medium containing octopine as the only added nitrogen source with bromothymolblue (BTB) as the pH indicator. On blue BTB plates, this strain formed orange-yellow colonies, while those formed by non-utilisers were translucent. This process of cell transformation may facilitate the transfer of genes of economic importance to rubber.
KW  - HEVEA BRASILIENSIS
KW  - AGROBACTERIUM TUMEFACIENS
KW  - GALLE/ EXPERIMENTATION IN VIVO
KW  - EXPERIMENTATION IN VITRO
KW  - TRANSFORMATION GENETIQUE
KW  - AGALLAS/ EXPERIMENTACION IN VIVO
KW  - EXPERIMENTACION IN VITRO
KW  - TRANSFORMACION GENETICA
ER  -