Improving of pelB-Secreted MPT64 protein released by Escherichia coli BL21 (DE3) using Triton X-100 and Tween-80
pelB has been known as a successful signal peptide to translocate the protein target extracellularly in the Escherichia coli system. However, in our previous study, the yield of MPT64 protein extracellular recovery was still low and plenty of this protein was remain trapped in cytoplasm and periplas...
Shranjeno v:
| Main Authors: | Kusuma, Sri Agung Fitri, Subroto, Toto, Parwati, Ida, Rukayadi, Yaya, Fadhlillah, Muhammad, Pardede, Ruth Michellee |
|---|---|
| Format: | Article |
| Izdano: |
Wolters Kluwer Medknow Publications
2022
|
| Oznake: |
Označite
Brez oznak, prvi označite!
|
Podobne knjige/članki
-
Optimization of culture conditions for Mpt64 synthetic gene expression in Escherichia coli BL21 (DE3) using surface response methodology
od: Kusuma, Sri Agung Fitri, et al.
Izdano: (2019) -
Construction and expression of synthetic gene encoding MPT64 as extracellular protein in Escherichia coli BL21 (DE3) expression system
od: Kusuma, Sri Agung Fitri, et al.
Izdano: (2018) -
Improvement of extracellular secretion efficiency of recombinant protein from Escherichia coli: signal peptide fusion, surfactants addition, and phospholipase
od: Kusuma, Sri Agung Fitri, et al.
Izdano: (2019) -
Triton paling kreatif
od: Osman, Hamzah
Izdano: (2013) -
Universal 64
od: AGCO GSI (Malaysia) Sdn Bhd, .
