Media composition to revive leptospires stored at −80 °C

Leptospires are preserved by frequent sub-culturing in semisolid media due to the challenge of low recovery by freezing or liquid nitrogen methods. The present study evaluated three liquid EMJH medium compositions (Medium A: Leptospira medium base EMJH, Leptospira enrichment EMJH, 5-fluorouracil (3%...

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Main Authors: Philip, Noraini, Neela, Vasantha Kumari
Formáid: Article
Foilsithe: Academic Press 2022
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